Detailed information of TA system TA127964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3067052..3067836
• Replicon: chromosome
• Accession: NZ_CP041300
• Organism: Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: FK541_RS15960
• Protein ID: WP_138031535.1
• Coordinates: 3067342..3067836 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A0A1AEM7
• Locus tag: FK541_RS15955
• Protein ID: WP_024946541.1
• Coordinates: 3067052..3067345 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK541_RS15945	3062213..3063172	-	960	WP_022645527.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
FK541_RS15950	3063745..3066918	+	3174	WP_063814920.1	ribonuclease E	-
FK541_RS15955	3067052..3067345	+	294	WP_024946541.1	DUF1778 domain-containing protein	Antitoxin
FK541_RS15960	3067342..3067836	+	495	WP_138031535.1	GNAT family N-acetyltransferase	Toxin
FK541_RS15965	3067931..3068884	-	954	WP_022645524.1	flagellar hook-associated protein FlgL	-
FK541_RS15970	3068896..3070539	-	1644	WP_022645523.1	flagellar hook-associated protein FlgK	-
FK541_RS15975	3070605..3071546	-	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
FK541_RS15980	3071546..3072643	-	1098	WP_021580666.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18030.95 Da
• Isoelectric Point: 7.6681

>T127964 WP_138031535.1 NZ_CP041300:3067342-3067836 [Escherichia coli O1:H42]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFCRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T127964 NZ_CP041300:3067342-3067836 [Escherichia coli O1:H42]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTTGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11039.60 Da
• Isoelectric Point: 10.5423

>AT127964 WP_024946541.1 NZ_CP041300:3067052-3067345 [Escherichia coli O1:H42]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT127964 NZ_CP041300:3067052-3067345 [Escherichia coli O1:H42]
ATGGCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATAGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCTCCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0A1AEM7