Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2663420..2664058 Replicon chromosome
Accession NZ_CP041300
Organism Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FK541_RS13915 Protein ID WP_000813794.1
Coordinates 2663882..2664058 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FK541_RS13910 Protein ID WP_001270286.1
Coordinates 2663420..2663836 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK541_RS13890 2658572..2659513 - 942 WP_022645667.1 ABC transporter permease -
FK541_RS13895 2659514..2660527 - 1014 WP_022645666.1 ABC transporter ATP-binding protein -
FK541_RS13900 2660545..2661690 - 1146 WP_000047466.1 ABC transporter substrate-binding protein -
FK541_RS13905 2661935..2663341 - 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
FK541_RS13910 2663420..2663836 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FK541_RS13915 2663882..2664058 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FK541_RS13920 2664280..2664510 + 231 WP_000491567.1 DUF2554 family protein -
FK541_RS13925 2664602..2666563 - 1962 WP_023909195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FK541_RS13930 2666636..2667172 - 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
FK541_RS13935 2667225..2668436 + 1212 WP_071788120.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T127963 WP_000813794.1 NZ_CP041300:c2664058-2663882 [Escherichia coli O1:H42]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T127963 NZ_CP041300:c2664058-2663882 [Escherichia coli O1:H42]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT127963 WP_001270286.1 NZ_CP041300:c2663836-2663420 [Escherichia coli O1:H42]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT127963 NZ_CP041300:c2663836-2663420 [Escherichia coli O1:H42]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References