Detailed information of TA system TA127952

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 791985..792742
• Replicon: chromosome
• Accession: NZ_CP041300
• Organism: Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TJC5
• Locus tag: FK541_RS04945
• Protein ID: WP_001094452.1
• Coordinates: 792368..792742 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8S7WZL4
• Locus tag: FK541_RS04940
• Protein ID: WP_071788590.1
• Coordinates: 791985..792278 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK541_RS04920	789660..790478	+	819	WP_001564116.1	DUF945 domain-containing protein	-
FK541_RS04925	790570..791055	+	486	WP_000206664.1	antirestriction protein	-
FK541_RS04930	791070..791546	+	477	WP_001186165.1	RadC family protein	-
FK541_RS04935	791609..791830	+	222	WP_000692345.1	DUF987 domain-containing protein	-
FK541_RS04940	791985..792278	+	294	WP_071788590.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FK541_RS04945	792368..792742	+	375	WP_001094452.1	TA system toxin CbtA family protein	Toxin
FK541_RS04950	792739..793230	+	492	WP_000976868.1	hypothetical protein	-
FK541_RS04955	793242..793439	+	198	WP_000772035.1	DUF957 domain-containing protein	-
FK541_RS04960	793536..794381	+	846	WP_001290254.1	DUF4942 domain-containing protein	-
FK541_RS04970	794680..795186	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
FK541_RS04975	795265..797106	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (1-92)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14014.86 Da
• Isoelectric Point: 7.2920

>T127952 WP_001094452.1 NZ_CP041300:792368-792742 [Escherichia coli O1:H42]
MNTLPDTHVREASRCSSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSTDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T127952 NZ_CP041300:792368-792742 [Escherichia coli O1:H42]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCTCATCTCCCGTAACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCACCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10804.40 Da
• Isoelectric Point: 8.2692

>AT127952 WP_071788590.1 NZ_CP041300:791985-792278 [Escherichia coli O1:H42]
VTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKADTLG
SCGYVYLAVYPTSETKK

Nucleotide

• Length: 294 bp

>AT127952 NZ_CP041300:791985-792278 [Escherichia coli O1:H42]
GTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGG
CCGGTTCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCA
GCGGTGAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAAAGGGCTGACCTGCAAAGCCGACACCCTCGGT
AGTTGTGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7Z7YIM6