Detailed information of TA system TA127949

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 71566..72401
• Replicon: chromosome
• Accession: NZ_CP041300
• Organism: Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TIK8
• Locus tag: FK541_RS01415
• Protein ID: WP_000854694.1
• Coordinates: 71566..71943 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: F4TIK7
• Locus tag: FK541_RS01420
• Protein ID: WP_001285605.1
• Coordinates: 72033..72401 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK541_RS01385	67205..68128	-	924	WP_022646286.1	carboxylate/amino acid/amine transporter	-
FK541_RS01390	68239..69423	-	1185	WP_001172866.1	sugar efflux transporter	-
FK541_RS01395	69807..69953	-	147	WP_001328030.1	hypothetical protein	-
FK541_RS01400	70220..71062	-	843	WP_001696593.1	DUF4942 domain-containing protein	-
FK541_RS01405	71147..71344	-	198	WP_086795284.1	DUF957 domain-containing protein	-
FK541_RS01410	71420..71569	-	150	Protein_59	hypothetical protein	-
FK541_RS01415	71566..71943	-	378	WP_000854694.1	TA system toxin CbtA family protein	Toxin
FK541_RS01420	72033..72401	-	369	WP_001285605.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FK541_RS01425	72481..72702	-	222	Protein_62	DUF987 domain-containing protein	-
FK541_RS01430	72789..73265	-	477	WP_001696592.1	RadC family protein	-
FK541_RS01435	73281..73760	-	480	WP_001696591.1	antirestriction protein	-
FK541_RS01440	74026..74844	-	819	WP_001234631.1	DUF945 domain-containing protein	-
FK541_RS01450	74943..75176	-	234	WP_001278287.1	DUF905 family protein	-
FK541_RS01455	75182..75859	-	678	WP_001097301.1	hypothetical protein	-
FK541_RS01460	76007..76687	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	60071..134114	74043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14008.02 Da
• Isoelectric Point: 7.7293

>T127949 WP_000854694.1 NZ_CP041300:c71943-71566 [Escherichia coli O1:H42]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T127949 NZ_CP041300:c71943-71566 [Escherichia coli O1:H42]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.48 Da
• Isoelectric Point: 6.0601

>AT127949 WP_001285605.1 NZ_CP041300:c72401-72033 [Escherichia coli O1:H42]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRACIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT127949 NZ_CP041300:c72401-72033 [Escherichia coli O1:H42]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCTGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2F934

Antitoxin

• AlphaFold DB: A0A2U2V3N6