Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 175234..175759 Replicon plasmid pCys-1
Accession NZ_CP041299
Organism Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FK541_RS00990 Protein ID WP_001159871.1
Coordinates 175454..175759 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag FK541_RS00985 Protein ID WP_000813630.1
Coordinates 175234..175452 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK541_RS00950 170628..171044 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
FK541_RS00955 171041..171271 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FK541_RS00960 171536..172036 + 501 WP_000528932.1 hypothetical protein -
FK541_RS00965 172049..172822 + 774 WP_000905949.1 hypothetical protein -
FK541_RS00970 172989..174122 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
FK541_RS00975 174156..174668 - 513 WP_000151784.1 hypothetical protein -
FK541_RS00985 175234..175452 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FK541_RS00990 175454..175759 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FK541_RS00995 175760..176565 + 806 Protein_174 tyrosine-type recombinase/integrase -
FK541_RS01000 177245..177976 - 732 WP_000504262.1 replication initiation protein -
FK541_RS01005 178597..179802 + 1206 WP_001442122.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucB / iucC / iucD / iutA 1..195702 195702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T127944 WP_001159871.1 NZ_CP041299:175454-175759 [Escherichia coli O1:H42]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T127944 NZ_CP041299:175454-175759 [Escherichia coli O1:H42]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT127944 WP_000813630.1 NZ_CP041299:175234-175452 [Escherichia coli O1:H42]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT127944 NZ_CP041299:175234-175452 [Escherichia coli O1:H42]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References