Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 170628..171271 Replicon plasmid pCys-1
Accession NZ_CP041299
Organism Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag FK541_RS00950 Protein ID WP_001034046.1
Coordinates 170628..171044 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag FK541_RS00955 Protein ID WP_001261278.1
Coordinates 171041..171271 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK541_RS00935 165765..166181 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
FK541_RS00940 166178..166408 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FK541_RS00945 166789..170583 + 3795 WP_171003983.1 pcar -
FK541_RS00950 170628..171044 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
FK541_RS00955 171041..171271 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FK541_RS00960 171536..172036 + 501 WP_000528932.1 hypothetical protein -
FK541_RS00965 172049..172822 + 774 WP_000905949.1 hypothetical protein -
FK541_RS00970 172989..174122 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
FK541_RS00975 174156..174668 - 513 WP_000151784.1 hypothetical protein -
FK541_RS00985 175234..175452 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
FK541_RS00990 175454..175759 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucB / iucC / iucD / iutA 1..195702 195702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T127943 WP_001034046.1 NZ_CP041299:c171044-170628 [Escherichia coli O1:H42]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T127943 NZ_CP041299:c171044-170628 [Escherichia coli O1:H42]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT127943 WP_001261278.1 NZ_CP041299:c171271-171041 [Escherichia coli O1:H42]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT127943 NZ_CP041299:c171271-171041 [Escherichia coli O1:H42]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References