Detailed information of TA system TA127941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 99059..99435
• Replicon: plasmid pCys-1
• Accession: NZ_CP041299
• Organism: Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: FK541_RS00610
• Protein ID: WP_000969988.1
• Coordinates: 99253..99435 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: FK541_RS00605
• Protein ID: WP_001324224.1
• Coordinates: 99059..99256 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK541_RS00585	94319..94555	+	237	WP_001323890.1	colicin V immunity protein	-
FK541_RS00590	94533..94844	+	312	WP_001259758.1	colicin V	-
FK541_RS00595	95014..97128	-	2115	WP_001183603.1	peptidase domain-containing ABC transporter	-
FK541_RS00600	97103..98377	-	1275	WP_000489608.1	HlyD family secretion protein	-
FK541_RS00605	99059..99256	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
FK541_RS00610	99253..99435	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FK541_RS00615	99534..99842	+	309	WP_000377483.1	hypothetical protein	-
FK541_RS00625	100402..101436	+	1035	WP_001190233.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucB / iucC / iucD / iutA	1..195702	195702

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T127941 WP_000969988.1 NZ_CP041299:99253-99435 [Escherichia coli O1:H42]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T127941 NZ_CP041299:99253-99435 [Escherichia coli O1:H42]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT127941 WP_001324224.1 NZ_CP041299:99059-99256 [Escherichia coli O1:H42]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT127941 NZ_CP041299:99059-99256 [Escherichia coli O1:H42]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3