Detailed information of TA system TA127940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 42051..42586
• Replicon: plasmid pCys-1
• Accession: NZ_CP041299
• Organism: Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: FK541_RS00260
• Protein ID: WP_000222760.1
• Coordinates: 42299..42586 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: FK541_RS00255
• Protein ID: WP_001132900.1
• Coordinates: 42051..42302 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK541_RS00245	37626..38186	+	561	WP_001161490.1	recombinase family protein	-
FK541_RS00250	38190..41156	+	2967	WP_138031477.1	Tn3-like element TnAs1 family transposase	-
FK541_RS00255	42051..42302	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FK541_RS00260	42299..42586	+	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FK541_RS00270	43026..43373	-	348	Protein_47	IS1-like element IS1A family transposase	-
FK541_RS00275	43462..44484	+	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
FK541_RS00280	44481..45263	+	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
FK541_RS00285	45318..45682	-	365	Protein_50	IS1 family transposase	-
FK541_RS00290	45782..47003	+	1222	Protein_51	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucB / iucC / iucD / iutA	1..195702	195702
	-	inside	IScluster/Tn	-	-	37626..46741	9115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T127940 WP_000222760.1 NZ_CP041299:42299-42586 [Escherichia coli O1:H42]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T127940 NZ_CP041299:42299-42586 [Escherichia coli O1:H42]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT127940 WP_001132900.1 NZ_CP041299:42051-42302 [Escherichia coli O1:H42]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT127940 NZ_CP041299:42051-42302 [Escherichia coli O1:H42]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2