Detailed information of TA system TA127935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 157..745
• Replicon: plasmid p80-1-2-p3
• Accession: NZ_CP041294
• Organism: Acinetobacter indicus strain 80-1-2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FK538_RS00035
• Protein ID: WP_168370255.1
• Coordinates: 458..745 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FK538_RS00030
• Protein ID: WP_168370254.1
• Coordinates: 157..471 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK538_RS00030	157..471	-	315	WP_168370254.1	BrnA antitoxin family protein	Antitoxin
FK538_RS00035	458..745	-	288	WP_168370255.1	BrnT family toxin	Toxin
FK538_RS00040	1054..1365	+	312	WP_168370256.1	3-hydroxybutyryl-CoA dehydrogenase	-
FK538_RS00045	1514..2722	-	1209	WP_160241796.1	IS256 family transposase	-
FK538_RS00050	2922..3464	-	543	WP_004967302.1	plasmid replication DNA-binding protein	-
FK538_RS00055	3594..4601	-	1008	WP_168370257.1	replication initiation protein	-
FK538_RS00060	5267..5470	+	204	WP_104472883.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9663	9663
	-	flank	IS/Tn	-	-	1514..2755	1241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11289.69 Da
• Isoelectric Point: 6.0872

>T127935 WP_168370255.1 NZ_CP041294:c745-458 [Acinetobacter indicus]
MEQYFEWDEAKNRKNQKKHDISFETASLVFDDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T127935 NZ_CP041294:c745-458 [Acinetobacter indicus]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTCTTTGATGATCCATTACGGATCTCAATCCAGGATAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACAATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12281.94 Da
• Isoelectric Point: 9.4865

>AT127935 WP_168370254.1 NZ_CP041294:c471-157 [Acinetobacter indicus]
MSMVRYSRKELNEKFSEKQNAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKSQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT127935 NZ_CP041294:c471-157 [Acinetobacter indicus]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTTAGTGAGAAGCAAAATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
ATAATCAGTTCTACCGTCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAATCTCAA
GGCAAAGGTTATCAAACACGAATGAATGAAATCTTGCGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA