Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 65107..65629 Replicon plasmid p54-tetX
Accession NZ_CP041286
Organism Escherichia coli strain 54

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag FK536_RS22855 Protein ID WP_000220560.1
Coordinates 65348..65629 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag FK536_RS22850 Protein ID WP_000121743.1
Coordinates 65107..65358 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK536_RS22825 60778..61059 + 282 WP_001185482.1 hypothetical protein -
FK536_RS22830 61084..62061 + 978 WP_024245156.1 hypothetical protein -
FK536_RS22835 62058..62483 - 426 WP_000202942.1 hypothetical protein -
FK536_RS22840 62551..62997 - 447 Protein_76 hypothetical protein -
FK536_RS22845 63037..63873 - 837 WP_001050931.1 replication initiation protein -
FK536_RS22850 65107..65358 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
FK536_RS22855 65348..65629 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FK536_RS22860 65704..66401 + 698 Protein_80 IS1 family transposase -
FK536_RS22865 66547..66804 + 258 WP_105278499.1 hypothetical protein -
FK536_RS22870 66841..67809 - 969 WP_077783444.1 IS5 family transposase -
FK536_RS22875 67844..68119 + 276 Protein_83 peptidoglycan DD-metalloendopeptidase family protein -
FK536_RS22880 68153..68350 - 198 WP_000843497.1 DUF2933 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS1 / tet(M) / blaTEM-1B / tet(A) / floR / tet(X4) / dfrA5 / sul3 / mef(B) - 1..102347 102347
- flank IS/Tn - - 66841..67809 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T127932 WP_000220560.1 NZ_CP041286:65348-65629 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T127932 NZ_CP041286:65348-65629 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT127932 WP_000121743.1 NZ_CP041286:65107-65358 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT127932 NZ_CP041286:65107-65358 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References