127911

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 21926..23034 Replicon plasmid pVVEswe-S4
Accession NZ_CP041279
Organism Enterococcus faecium strain VVEswe-S

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag FLC01_RS16040 Protein ID WP_002303392.1
Coordinates 21926..22795 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag FLC01_RS16045 Protein ID WP_002303393.1
Coordinates 22810..23034 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLC01_RS16010 17219..17470 - 252 Protein_23 GNAT family N-acetyltransferase -
FLC01_RS16015 17715..19010 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
FLC01_RS16020 19111..20019 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
FLC01_RS16025 20022..20270 - 249 WP_002347175.1 hypothetical protein -
FLC01_RS16030 20267..21175 - 909 WP_060810973.1 aminoglycoside 6-adenylyltransferase -
FLC01_RS16035 21208..21945 - 738 WP_060810974.1 methyltransferase domain-containing protein -
FLC01_RS16040 21926..22795 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
FLC01_RS16045 22810..23034 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
FLC01_RS16050 23177..23998 - 822 Protein_31 recombinase zinc beta ribbon domain-containing protein -
FLC01_RS16055 24686..25174 - 489 Protein_32 lincosamide nucleotidyltransferase Lnu(B) -
FLC01_RS16060 25229..25909 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
FLC01_RS16065 26158..26676 + 519 WP_000357244.1 YfbU family protein -
FLC01_RS16070 26683..27195 + 513 WP_000774078.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) / aph(3')-III / ant(6)-Ia - 1..34466 34466
- inside IScluster/Tn VanHAX / erm(B) / aph(3')-III / ant(6)-Ia - 1949..31841 29892


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T127911 WP_002303392.1 NZ_CP041279:c22795-21926 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T127911 NZ_CP041279:c22795-21926 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAAGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAGTTGGATGATGAGAATAGAAACAACCTTGTTGTACCTCCCGGAGCGTGGGGTGATTGG
GTTAATGGCGGTGGATGGTTAGTTATAAACGGGTGTCATGTTGACTTGATATTACGTGATATAAAACGGGTGGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT127911 WP_002303393.1 NZ_CP041279:c23034-22810 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT127911 NZ_CP041279:c23034-22810 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTTAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References