Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 13445..14582 Replicon plasmid pVVEswe-S4
Accession NZ_CP041279
Organism Enterococcus faecium strain VVEswe-S

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag FLC01_RS15985 Protein ID WP_001284311.1
Coordinates 13719..14582 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag FLC01_RS15980 Protein ID WP_000301765.1
Coordinates 13445..13717 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLC01_RS15940 8692..9345 - 654 WP_146419990.1 VanR-ABDEGLN family response regulator transcription factor -
FLC01_RS15945 9387..10067 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
FLC01_RS15950 10141..10461 - 321 Protein_11 RepB family plasmid replication initiator protein -
FLC01_RS15955 11114..11209 + 96 WP_106913803.1 type I toxin-antitoxin system Fst family toxin -
FLC01_RS15960 11512..11757 - 246 WP_002311817.1 hypothetical protein -
FLC01_RS15965 12244..12924 + 681 WP_002328852.1 IS6-like element IS1216 family transposase -
FLC01_RS15970 12978..13121 + 144 Protein_15 chromosome partitioning protein ParA -
FLC01_RS15975 13219..13428 + 210 WP_000527318.1 peptide-binding protein -
FLC01_RS15980 13445..13717 + 273 WP_000301765.1 antitoxin Antitoxin
FLC01_RS15985 13719..14582 + 864 WP_001284311.1 toxin zeta Toxin
FLC01_RS15990 14845..15582 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
FLC01_RS15995 15707..15790 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
FLC01_RS16000 15839..15922 - 84 Protein_21 peptide-binding protein -
FLC01_RS16005 16332..17126 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
FLC01_RS16010 17219..17470 - 252 Protein_23 GNAT family N-acetyltransferase -
FLC01_RS16015 17715..19010 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) / aph(3')-III / ant(6)-Ia - 1..34466 34466
- inside IScluster/Tn VanHAX / erm(B) / aph(3')-III / ant(6)-Ia - 1949..31841 29892


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T127910 WP_001284311.1 NZ_CP041279:13719-14582 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T127910 NZ_CP041279:13719-14582 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT127910 WP_000301765.1 NZ_CP041279:13445-13717 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT127910 NZ_CP041279:13445-13717 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References