Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 21883..22991 Replicon plasmid pVVEswe-R4
Accession NZ_CP041269
Organism Enterococcus faecium strain VVEswe-R

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag FKW92_RS16070 Protein ID WP_002303392.1
Coordinates 21883..22752 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag FKW92_RS16075 Protein ID WP_002303393.1
Coordinates 22767..22991 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKW92_RS16040 17176..17427 - 252 Protein_23 GNAT family N-acetyltransferase -
FKW92_RS16045 17672..18967 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
FKW92_RS16050 19068..19976 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
FKW92_RS16055 19979..20227 - 249 WP_002347175.1 hypothetical protein -
FKW92_RS16060 20224..21132 - 909 WP_060810973.1 aminoglycoside 6-adenylyltransferase -
FKW92_RS16065 21165..21902 - 738 WP_060810974.1 methyltransferase domain-containing protein -
FKW92_RS16070 21883..22752 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
FKW92_RS16075 22767..22991 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
FKW92_RS16080 23134..23955 - 822 Protein_31 recombinase zinc beta ribbon domain-containing protein -
FKW92_RS16085 24643..25131 - 489 Protein_32 lincosamide nucleotidyltransferase Lnu(B) -
FKW92_RS16090 25186..25866 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
FKW92_RS16095 26115..26633 + 519 WP_000357244.1 YfbU family protein -
FKW92_RS16100 26640..27152 + 513 WP_000774078.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) / aph(3')-III / ant(6)-Ia - 1..35172 35172
- inside IScluster/Tn VanHAX / erm(B) / aph(3')-III / ant(6)-Ia - 1950..32548 30598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T127905 WP_002303392.1 NZ_CP041269:c22752-21883 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T127905 NZ_CP041269:c22752-21883 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAAGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAGTTGGATGATGAGAATAGAAACAACCTTGTTGTACCTCCCGGAGCGTGGGGTGATTGG
GTTAATGGCGGTGGATGGTTAGTTATAAACGGGTGTCATGTTGACTTGATATTACGTGATATAAAACGGGTGGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT127905 WP_002303393.1 NZ_CP041269:c22991-22767 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT127905 NZ_CP041269:c22991-22767 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTTAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References