Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 13402..14539 Replicon plasmid pVVEswe-R4
Accession NZ_CP041269
Organism Enterococcus faecium strain VVEswe-R

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag FKW92_RS16015 Protein ID WP_001284311.1
Coordinates 13676..14539 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag FKW92_RS16010 Protein ID WP_000301765.1
Coordinates 13402..13674 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKW92_RS15970 8649..9302 - 654 WP_146419990.1 VanR-ABDEGLN family response regulator transcription factor -
FKW92_RS15975 9344..10024 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
FKW92_RS15980 10098..10418 - 321 Protein_11 RepB family plasmid replication initiator protein -
FKW92_RS15985 11071..11166 + 96 WP_106913803.1 type I toxin-antitoxin system Fst family toxin -
FKW92_RS15990 11469..11714 - 246 WP_002311817.1 hypothetical protein -
FKW92_RS15995 12201..12881 + 681 WP_002328852.1 IS6-like element IS1216 family transposase -
FKW92_RS16000 12935..13078 + 144 Protein_15 chromosome partitioning protein ParA -
FKW92_RS16005 13176..13385 + 210 WP_000527318.1 peptide-binding protein -
FKW92_RS16010 13402..13674 + 273 WP_000301765.1 antitoxin Antitoxin
FKW92_RS16015 13676..14539 + 864 WP_001284311.1 toxin zeta Toxin
FKW92_RS16020 14802..15539 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
FKW92_RS16025 15664..15747 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
FKW92_RS16030 15796..15879 - 84 Protein_21 peptide-binding protein -
FKW92_RS16035 16289..17083 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
FKW92_RS16040 17176..17427 - 252 Protein_23 GNAT family N-acetyltransferase -
FKW92_RS16045 17672..18967 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) / aph(3')-III / ant(6)-Ia - 1..35172 35172
- inside IScluster/Tn VanHAX / erm(B) / aph(3')-III / ant(6)-Ia - 1950..32548 30598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T127904 WP_001284311.1 NZ_CP041269:13676-14539 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T127904 NZ_CP041269:13676-14539 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT127904 WP_000301765.1 NZ_CP041269:13402-13674 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT127904 NZ_CP041269:13402-13674 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References