Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 474971..475542 Replicon chromosome
Accession NZ_CP041261
Organism Enterococcus faecium strain VVEswe-R

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag FKW92_RS02235 Protein ID WP_002286801.1
Coordinates 475201..475542 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag FKW92_RS02230 Protein ID WP_002323011.1
Coordinates 474971..475201 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKW92_RS02200 470107..471435 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
FKW92_RS02205 471457..472083 + 627 WP_002286816.1 cysteine hydrolase -
FKW92_RS02210 472266..472847 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
FKW92_RS02215 473579..473926 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
FKW92_RS02220 473902..474141 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
FKW92_RS02225 474346..474684 - 339 WP_002286804.1 hypothetical protein -
FKW92_RS02230 474971..475201 + 231 WP_002323011.1 hypothetical protein Antitoxin
FKW92_RS02235 475201..475542 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FKW92_RS02240 476392..476577 + 186 WP_002304207.1 hypothetical protein -
FKW92_RS02245 477082..477276 + 195 WP_002297028.1 hypothetical protein -
FKW92_RS02250 477434..477716 + 283 Protein_446 transposase -
FKW92_RS02255 477960..478790 - 831 WP_002286789.1 manganese catalase family protein -
FKW92_RS02260 478998..480332 + 1335 WP_002303373.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T127901 WP_002286801.1 NZ_CP041261:475201-475542 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T127901 NZ_CP041261:475201-475542 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT127901 WP_002323011.1 NZ_CP041261:474971-475201 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT127901 NZ_CP041261:474971-475201 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References