Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 33364..33971 Replicon plasmid p27
Accession NZ_CP041258
Organism Enterococcus faecium strain 515

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag FKZ20_RS15970 Protein ID WP_002321032.1
Coordinates 33364..33714 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag FKZ20_RS15975 Protein ID WP_002287514.1
Coordinates 33708..33971 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKZ20_RS15925 29005..29088 + 84 Protein_39 peptide-binding protein -
FKZ20_RS15930 29137..29220 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
FKZ20_RS15935 29345..30082 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
FKZ20_RS15940 30345..31208 - 864 WP_001284311.1 toxin zeta -
FKZ20_RS15945 31210..31482 - 273 WP_000301765.1 antitoxin -
FKZ20_RS15950 31499..31708 - 210 WP_000527318.1 peptide-binding protein -
FKZ20_RS15955 31806..32000 - 195 Protein_45 chromosome partitioning protein ParA -
FKZ20_RS15960 32055..32735 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
FKZ20_RS15965 32780..33340 + 561 WP_080386592.1 recombinase family protein -
FKZ20_RS15970 33364..33714 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FKZ20_RS15975 33708..33971 - 264 WP_002287514.1 hypothetical protein Antitoxin
FKZ20_RS15980 34284..34463 - 180 WP_002298867.1 hypothetical protein -
FKZ20_RS15990 34908..35345 - 438 WP_002326886.1 hypothetical protein -
FKZ20_RS15995 35361..35612 - 252 WP_002299286.1 hypothetical protein -
FKZ20_RS16000 35612..35818 - 207 WP_002296239.1 hypothetical protein -
FKZ20_RS16010 36265..36525 - 261 WP_002296240.1 hypothetical protein -
FKZ20_RS16015 36891..37388 - 498 WP_002326885.1 single-stranded DNA-binding protein -
FKZ20_RS16020 37391..38290 - 900 WP_002326884.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
FKZ20_RS16025 38303..38896 - 594 WP_002326883.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1..81972 81972
- inside IScluster/Tn lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 6310..32741 26431


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T127899 WP_002321032.1 NZ_CP041258:c33714-33364 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T127899 NZ_CP041258:c33714-33364 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
GCCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT127899 WP_002287514.1 NZ_CP041258:c33971-33708 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT127899 NZ_CP041258:c33971-33708 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References