Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 30345..31482 Replicon plasmid p27
Accession NZ_CP041258
Organism Enterococcus faecium strain 515

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag FKZ20_RS15940 Protein ID WP_001284311.1
Coordinates 30345..31208 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag FKZ20_RS15945 Protein ID WP_000301765.1
Coordinates 31210..31482 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKZ20_RS15910 25917..27212 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
FKZ20_RS15915 27457..27708 + 252 Protein_37 GNAT family N-acetyltransferase -
FKZ20_RS15920 27801..28595 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
FKZ20_RS15925 29005..29088 + 84 Protein_39 peptide-binding protein -
FKZ20_RS15930 29137..29220 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
FKZ20_RS15935 29345..30082 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
FKZ20_RS15940 30345..31208 - 864 WP_001284311.1 toxin zeta Toxin
FKZ20_RS15945 31210..31482 - 273 WP_000301765.1 antitoxin Antitoxin
FKZ20_RS15950 31499..31708 - 210 WP_000527318.1 peptide-binding protein -
FKZ20_RS15955 31806..32000 - 195 Protein_45 chromosome partitioning protein ParA -
FKZ20_RS15960 32055..32735 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
FKZ20_RS15965 32780..33340 + 561 WP_080386592.1 recombinase family protein -
FKZ20_RS15970 33364..33714 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin -
FKZ20_RS15975 33708..33971 - 264 WP_002287514.1 hypothetical protein -
FKZ20_RS15980 34284..34463 - 180 WP_002298867.1 hypothetical protein -
FKZ20_RS15990 34908..35345 - 438 WP_002326886.1 hypothetical protein -
FKZ20_RS15995 35361..35612 - 252 WP_002299286.1 hypothetical protein -
FKZ20_RS16000 35612..35818 - 207 WP_002296239.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1..81972 81972
- inside IScluster/Tn lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 6310..32741 26431


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T127898 WP_001284311.1 NZ_CP041258:c31208-30345 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T127898 NZ_CP041258:c31208-30345 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT127898 WP_000301765.1 NZ_CP041258:c31482-31210 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT127898 NZ_CP041258:c31482-31210 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References