127897

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 21896..23004 Replicon plasmid p27
Accession NZ_CP041258
Organism Enterococcus faecium strain 515

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag FKZ20_RS15885 Protein ID WP_002303392.1
Coordinates 22135..23004 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag FKZ20_RS15880 Protein ID WP_002303393.1
Coordinates 21896..22120 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKZ20_RS15850 17004..17516 - 513 WP_000774078.1 hypothetical protein -
FKZ20_RS15855 17523..18041 - 519 WP_000357244.1 YfbU family protein -
FKZ20_RS15860 18203..18415 - 213 WP_002349227.1 hypothetical protein -
FKZ20_RS15865 18624..19304 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
FKZ20_RS15870 19441..20244 + 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
FKZ20_RS15875 20932..21753 + 822 Protein_29 recombinase zinc beta ribbon domain-containing protein -
FKZ20_RS15880 21896..22120 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
FKZ20_RS15885 22135..23004 + 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
FKZ20_RS15890 22985..23719 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
FKZ20_RS15895 23752..24660 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
FKZ20_RS15900 24657..24905 + 249 WP_002347175.1 hypothetical protein -
FKZ20_RS15905 24908..25816 - 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
FKZ20_RS15910 25917..27212 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
FKZ20_RS15915 27457..27708 + 252 Protein_37 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1..81972 81972
- inside IScluster/Tn lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 6310..32741 26431


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T127897 WP_002303392.1 NZ_CP041258:22135-23004 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T127897 NZ_CP041258:22135-23004 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAAGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAGTTGGATGATGAGAATAGAAACAACCTTGTTGTACCTCCCGGAGCGTGGGGTGATTGG
GTTAATGGCGGTGGATGGTTAGTTATAAACGGGTGTCATGTTGACTTGATATTACGTGATATAAAACGGGTGGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT127897 WP_002303393.1 NZ_CP041258:21896-22120 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT127897 NZ_CP041258:21896-22120 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTTAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References