Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2509011..2509582 Replicon chromosome
Accession NZ_CP041257
Organism Enterococcus faecium strain 515

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag FKZ20_RS14420 Protein ID WP_002286801.1
Coordinates 2509241..2509582 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag FKZ20_RS14415 Protein ID WP_002323011.1
Coordinates 2509011..2509241 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKZ20_RS14365 2504147..2505475 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
FKZ20_RS14370 2505497..2506123 + 627 WP_002286816.1 cysteine hydrolase -
FKZ20_RS14375 2506306..2506887 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
FKZ20_RS14395 2507619..2507966 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
FKZ20_RS14400 2507942..2508181 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
FKZ20_RS14405 2508386..2508724 - 339 WP_002286804.1 hypothetical protein -
FKZ20_RS14415 2509011..2509241 + 231 WP_002323011.1 hypothetical protein Antitoxin
FKZ20_RS14420 2509241..2509582 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FKZ20_RS14425 2510432..2510617 + 186 WP_002304207.1 hypothetical protein -
FKZ20_RS14430 2511122..2511316 + 195 WP_002297028.1 hypothetical protein -
FKZ20_RS14435 2511474..2511756 + 283 Protein_2360 transposase -
FKZ20_RS14445 2512000..2512830 - 831 WP_002286789.1 manganese catalase family protein -
FKZ20_RS14450 2513038..2514372 + 1335 WP_002303373.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T127896 WP_002286801.1 NZ_CP041257:2509241-2509582 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T127896 NZ_CP041257:2509241-2509582 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT127896 WP_002323011.1 NZ_CP041257:2509011-2509241 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT127896 NZ_CP041257:2509011-2509241 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References