Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 13921..14446 Replicon plasmid unnamed3
Accession NZ_CP041250
Organism Raoultella electrica strain DSM 102253

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag Electrica_RS27635 Protein ID WP_001568026.1
Coordinates 13921..14226 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag Electrica_RS27640 Protein ID WP_001568025.1
Coordinates 14228..14446 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Electrica_RS27615 10391..11659 + 1269 WP_142255980.1 ISL3 family transposase -
Electrica_RS27620 11994..13010 - 1017 WP_020315256.1 hypothetical protein -
Electrica_RS27625 13007..13330 - 324 WP_022644730.1 hypothetical protein -
Electrica_RS27630 13357..13752 - 396 WP_072158608.1 hypothetical protein -
Electrica_RS27635 13921..14226 - 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
Electrica_RS27640 14228..14446 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
Electrica_RS27645 15024..16106 + 1083 WP_023340900.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..83988 83988
- flank IS/Tn - - 10391..11659 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T127892 WP_001568026.1 NZ_CP041250:c14226-13921 [Raoultella electrica]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T127892 NZ_CP041250:c14226-13921 [Raoultella electrica]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT127892 WP_001568025.1 NZ_CP041250:c14446-14228 [Raoultella electrica]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT127892 NZ_CP041250:c14446-14228 [Raoultella electrica]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References