Detailed information of TA system TA127889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4582913..4583489
• Replicon: chromosome
• Accession: NZ_CP041247
• Organism: Raoultella electrica strain DSM 102253

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: Electrica_RS21920
• Protein ID: WP_131049471.1
• Coordinates: 4583202..4583489 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: Electrica_RS21915
• Protein ID: WP_141965380.1
• Coordinates: 4582913..4583215 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Electrica_RS21895	4578884..4579951	-	1068	WP_141965378.1	HlyD family secretion protein	-
Electrica_RS21900	4579955..4580419	-	465	WP_141965379.1	MarR family transcriptional regulator	-
Electrica_RS21905	4581018..4581182	-	165	WP_100684675.1	DUF1127 domain-containing protein	-
Electrica_RS21910	4581360..4582772	+	1413	WP_100684676.1	PLP-dependent aminotransferase family protein	-
Electrica_RS21915	4582913..4583215	-	303	WP_141965380.1	BrnA antitoxin family protein	Antitoxin
Electrica_RS21920	4583202..4583489	-	288	WP_131049471.1	BrnT family toxin	Toxin
Electrica_RS21925	4583824..4584267	-	444	WP_141965381.1	FosA family fosfomycin resistance glutathione transferase	-
Electrica_RS21930	4584261..4585169	-	909	WP_141965382.1	LysR family transcriptional regulator	-
Electrica_RS21935	4585257..4586039	+	783	WP_141965383.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11135.61 Da
• Isoelectric Point: 8.5787

>T127889 WP_131049471.1 NZ_CP041247:c4583489-4583202 [Raoultella electrica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRQDRYQNGEYRWQTLGLVHGIIVILVAHSVRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T127889 NZ_CP041247:c4583489-4583202 [Raoultella electrica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTGAGAAAACATGGGATTCGGTTTGAAGAGGCGGT
ACTAGTCTTTGACGATCCGCAGCATCTTTCCCGACAAGATCGCTATCAAAATGGTGAATATCGATGGCAAACCCTCGGCC
TTGTACACGGAATTATCGTTATTCTTGTTGCGCATTCGGTTCGTTTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCAGATAAGAAAGAGAGGAGTCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11564.11 Da
• Isoelectric Point: 9.7962

>AT127889 WP_141965380.1 NZ_CP041247:c4583215-4582913 [Raoultella electrica]
MNMVKHKRDNPAALTAQREDELKALAKKSDDDIDYSDIPSSDDEQWSEAVRGKFYRPLKTQASVRIDADVMAWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT127889 NZ_CP041247:c4583215-4582913 [Raoultella electrica]
ATGAACATGGTTAAACATAAACGCGATAACCCGGCCGCGCTTACCGCCCAGCGTGAAGACGAACTCAAAGCGCTGGCGAA
AAAATCAGATGATGACATTGATTACAGCGACATCCCCTCTTCGGACGATGAGCAGTGGTCAGAGGCGGTTCGCGGTAAAT
TTTATCGGCCGCTAAAAACGCAGGCCTCTGTGCGCATCGATGCTGATGTTATGGCGTGGCTTAAACGCCCGGGAAAAGGT
TATCAAACCCGGCTGAACGCGATTCTGCGGGAAGCCATGCTGCGCGAGCAGAATAAGAAATAA