Detailed information of TA system TA127886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2584655..2585406
• Replicon: chromosome
• Accession: NZ_CP041247
• Organism: Raoultella electrica strain DSM 102253

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Electrica_RS12375
• Protein ID: WP_141964594.1
• Coordinates: 2584924..2585406 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: Electrica_RS12370
• Protein ID: WP_100683705.1
• Coordinates: 2584655..2584933 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Electrica_RS12350	2580022..2581122	-	1101	WP_131050358.1	amino acid ABC transporter permease	-
Electrica_RS12355	2581138..2582316	-	1179	WP_131050357.1	amino acid ABC transporter permease	-
Electrica_RS12360	2582387..2583412	-	1026	WP_100683707.1	amino acid ABC transporter substrate-binding protein	-
Electrica_RS12365	2583973..2584575	+	603	WP_141964593.1	DJ-1/PfpI family protein	-
Electrica_RS12370	2584655..2584933	+	279	WP_100683705.1	DUF1778 domain-containing protein	Antitoxin
Electrica_RS12375	2584924..2585406	+	483	WP_141964594.1	GNAT family N-acetyltransferase	Toxin
Electrica_RS12380	2585457..2585993	+	537	WP_100683703.1	dihydrofolate reductase family protein	-
Electrica_RS12385	2586153..2586944	-	792	WP_141964595.1	tetratricopeptide repeat protein	-
Electrica_RS12390	2587051..2587497	-	447	Protein_2382	helix-turn-helix domain-containing protein	-
Electrica_RS12395	2587486..2588034	+	549	Protein_2383	DUF637 domain-containing protein	-
Electrica_RS12400	2588317..2588481	+	165	Protein_2384	SOS response-associated peptidase	-
Electrica_RS12405	2588735..2589634	-	900	WP_131050350.1	ABC transporter six-transmembrane domain-containing protein	-
Electrica_RS12410	2589722..2590318	+	597	WP_131050349.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2497474..2654021	156547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (75-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17681.43 Da
• Isoelectric Point: 9.1567

>T127886 WP_141964594.1 NZ_CP041247:2584924-2585406 [Raoultella electrica]
MGMRPPESLTPEHDIAEFCCQDPGLNEWLKKKALKNHSTGISRVYVVCAGNTNRVIAYYCLSSGSVHRNTVPGTYRRNAP
ESVPVIVQGRLAVDVSWAGKGLGAALLKDAIYRTQNIAVQVGVRALLVHALNDEVRDFYTRFGFEPSIVNVLTLLFPIRL

Nucleotide

• Length: 483 bp

>T127886 NZ_CP041247:2584924-2585406 [Raoultella electrica]
ATGGGAATGAGGCCCCCGGAATCACTCACCCCTGAACACGACATTGCTGAATTTTGTTGCCAGGACCCAGGTCTTAATGA
GTGGTTGAAGAAGAAAGCACTGAAAAACCACAGTACGGGCATTTCAAGGGTCTATGTGGTCTGTGCCGGGAACACAAACA
GGGTGATCGCGTATTACTGCTTGTCTTCCGGAAGTGTGCACCGAAACACAGTTCCTGGGACCTACCGTCGTAATGCACCT
GAGTCCGTACCGGTCATCGTTCAGGGAAGGCTTGCGGTCGATGTATCCTGGGCAGGAAAGGGACTCGGAGCTGCGCTGCT
TAAGGACGCCATATACCGCACCCAAAACATCGCAGTGCAGGTTGGCGTGAGGGCGCTTCTCGTCCACGCCCTGAATGATG
AAGTCAGGGATTTTTATACCCGGTTTGGTTTTGAGCCTTCTATTGTTAACGTTCTGACATTATTGTTCCCGATCAGACTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10740.28 Da
• Isoelectric Point: 4.5533

>AT127886 WP_100683705.1 NZ_CP041247:2584655-2584933 [Raoultella electrica]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFLLDDKQFESFIEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT127886 NZ_CP041247:2584655-2584933 [Raoultella electrica]
ATGAAACCGGACATCCGTGAAGCCCCGATAAATATTCGGGCAAAAACATCACAACGCGATCTTATCGACATGGCCGCAAA
CCTTGTATCAAAATCACGTACTGATTTTATGCTCGAGGCGGCCTGCCGCGAGGCCCAGGACATTCTGCTGGATCAGCGCT
TATTTCTGCTGGACGATAAGCAGTTTGAGTCATTTATTGAAGAGCTTGATGCCCCTATGACAGCGGAGCGCCAGGCGCGT
ATTGATAACCTGATGAACCGCAAATCGCCATGGGAATGA