Detailed information of TA system TA127875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 705080..705639
• Replicon: chromosome
• Accession: NZ_CP041247
• Organism: Raoultella electrica strain DSM 102253

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: Electrica_RS03420
• Protein ID: WP_141963447.1
• Coordinates: 705319..705639 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: Electrica_RS03415
• Protein ID: WP_141963445.1
• Coordinates: 705080..705316 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Electrica_RS03405	701960..703237	-	1278	WP_141963441.1	site-specific DNA-methyltransferase	-
Electrica_RS03410	703816..704760	+	945	WP_141963443.1	DUF1738 domain-containing protein	-
Electrica_RS03415	705080..705316	+	237	WP_141963445.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
Electrica_RS03420	705319..705639	+	321	WP_141963447.1	CcdB family protein	Toxin
Electrica_RS03425	705856..706197	-	342	WP_141963449.1	FxLYD domain-containing protein	-
Electrica_RS03430	706571..707233	-	663	WP_141963451.1	HAD-IB family hydrolase	-
Electrica_RS03435	707249..708000	-	752	Protein_666	hypothetical protein	-
Electrica_RS03440	708011..709216	-	1206	Protein_667	acetyl-CoA C-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	698142..721930	23788

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11820.76 Da
• Isoelectric Point: 7.9888

>T127875 WP_141963447.1 NZ_CP041247:705319-705639 [Raoultella electrica]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVDRYPAGRRPDRLVPVVRLTDGQEYAVMTHELASVPVRALG
VMFCDASQYRTQVKAAIDFLIDGIWS

Nucleotide

• Length: 321 bp

>T127875 NZ_CP041247:705319-705639 [Raoultella electrica]
ATGCAGTTCACCGTATACGGGAATACCGGTAAAAGCGCCGTTTACCCACTGTTGCTTGATGTCACAAGCGATATTATTGG
ACAACTGAACCGCCGGATCGTGATCCCGTTGCTGCCTGTTGACAGATATCCGGCAGGCCGCCGCCCGGATCGCCTTGTCC
CCGTGGTCAGACTGACGGACGGCCAGGAATACGCTGTCATGACTCACGAACTGGCGAGTGTCCCTGTCCGGGCGCTGGGG
GTGATGTTCTGTGATGCTTCGCAGTACCGCACTCAGGTAAAGGCCGCTATAGATTTCCTCATCGACGGCATTTGGTCGTG
A

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9018.00 Da
• Isoelectric Point: 6.2206

>AT127875 WP_141963445.1 NZ_CP041247:705080-705316 [Raoultella electrica]
MTAKQRSTQSVTMTVERALLSRAREAGINLSATLTTALDAELRHYEAKKWQEENREAIDALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT127875 NZ_CP041247:705080-705316 [Raoultella electrica]
ATGACAGCGAAACAACGCAGCACACAGAGTGTGACCATGACGGTAGAGCGAGCCTTACTGAGCCGGGCGCGTGAGGCAGG
CATTAACCTCAGTGCGACCCTGACAACCGCCCTCGATGCGGAGCTTCGCCACTATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAGGCCATCGACGCATTAAACCGTTTTCATGATGAACATGGCTGTTTCAGTGATGAATATAGGACATTTTAA