Detailed information of TA system TA127867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 151610..152139
• Replicon: plasmid pEmeITTGR7b
• Accession: NZ_CP041240
• Organism: Ensifer mexicanus strain ITTG R7

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FKV68_RS22900
• Protein ID: WP_180942302.1
• Coordinates: 151933..152139 (-)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FKV68_RS22895
• Protein ID: WP_180942250.1
• Coordinates: 151610..151936 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKV68_RS22875	147158..147685	+	528	WP_180942247.1	hypothetical protein	-
FKV68_RS22880	147710..149654	+	1945	Protein_133	acyltransferase	-
FKV68_RS22885	149680..149964	-	285	WP_180942248.1	omptin family outer membrane protease	-
FKV68_RS22890	150079..150378	-	300	WP_180942249.1	omptin family outer membrane protease	-
FKV68_RS22895	151610..151936	-	327	WP_180942250.1	BrnA antitoxin family protein	Antitoxin
FKV68_RS22900	151933..152139	-	207	WP_180942302.1	BrnT family toxin	Toxin
FKV68_RS22905	152617..153299	+	683	Protein_138	OmpW family protein	-
FKV68_RS22910	153365..153454	-	90	WP_180942251.1	hypothetical protein	-
FKV68_RS22915	153661..154158	+	498	WP_180942252.1	NUDIX hydrolase	-
FKV68_RS22920	154463..155572	-	1110	WP_180942253.1	FAD-dependent monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..455676	455676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (1-64)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7399.56 Da
• Isoelectric Point: 8.7102

>T127867 WP_180942302.1 NZ_CP041240:c152139-151933 [Ensifer mexicanus]
IAKHGYDFADLDMEFFASSVVVPAKEGRFTAIGVLRNGVIAVVFARLGSEGVSIISMRDANRKERSLL

Nucleotide

• Length: 207 bp

>T127867 NZ_CP041240:c152139-151933 [Ensifer mexicanus]
ATCGCCAAGCACGGCTATGATTTCGCCGATCTGGACATGGAATTTTTCGCTTCGTCTGTGGTGGTCCCGGCCAAGGAAGG
TCGCTTCACGGCGATCGGCGTTCTGCGCAATGGTGTGATTGCCGTAGTCTTCGCCCGCCTTGGTTCGGAAGGCGTTTCGA
TTATCTCCATGCGTGACGCCAACCGGAAGGAAAGGAGCTTACTATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12307.92 Da
• Isoelectric Point: 10.1665

>AT127867 WP_180942250.1 NZ_CP041240:c151936-151610 [Ensifer mexicanus]
MTNLSRRIRRISDKEEADIQRKIASDPDAPEATDEQLRQAKPFAEAFPDLAESIKRARGRPAVENPRQQISIRLDPDIID
YYKKNCGKGWQSRMNDDLRKAAGLKKAG

Nucleotide

• Length: 327 bp

>AT127867 NZ_CP041240:c151936-151610 [Ensifer mexicanus]
ATGACCAATCTATCCCGCCGCATCCGCCGGATCTCCGACAAGGAGGAGGCAGATATTCAGCGCAAGATAGCCAGCGACCC
AGACGCCCCAGAGGCCACCGACGAGCAGCTTAGGCAGGCAAAGCCTTTTGCTGAGGCTTTCCCGGACCTGGCCGAGAGCA
TCAAGCGTGCCAGAGGCCGACCGGCAGTTGAGAACCCAAGGCAGCAGATATCCATTCGCCTCGATCCGGATATCATTGAC
TATTACAAAAAGAACTGCGGCAAAGGCTGGCAAAGCCGGATGAACGACGACCTTAGGAAGGCTGCCGGGTTGAAGAAGGC
CGGTTAA