Detailed information of TA system TA127866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 30032..30630
• Replicon: plasmid pEmeITTGR7a
• Accession: NZ_CP041239
• Organism: Ensifer mexicanus strain ITTG R7

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FKV68_RS20290
• Protein ID: WP_180941787.1
• Coordinates: 30337..30630 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FKV68_RS20285
• Protein ID: WP_180941786.1
• Coordinates: 30032..30340 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKV68_RS20270	25124..27109	+	1986	WP_180941928.1	AAA family ATPase	-
FKV68_RS20275	27106..28404	+	1299	WP_180941784.1	restriction endonuclease	-
FKV68_RS20280	28480..29751	+	1272	WP_180941785.1	hypothetical protein	-
FKV68_RS20285	30032..30340	+	309	WP_180941786.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FKV68_RS20290	30337..30630	+	294	WP_180941787.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FKV68_RS20295	30665..31135	+	471	WP_180941788.1	hypothetical protein	-
FKV68_RS20300	31141..32010	-	870	WP_180941789.1	hypothetical protein	-
FKV68_RS20305	32134..32895	-	762	WP_180941790.1	DUF2726 domain-containing protein	-
FKV68_RS20310	33068..35419	-	2352	WP_180941791.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..436172	436172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11227.93 Da
• Isoelectric Point: 7.2040

>T127866 WP_180941787.1 NZ_CP041239:30337-30630 [Ensifer mexicanus]
MRLVWARYALDDRDTIFSRIESENPKAAIHVDEEIVRAARRLLDFPESGRPGRIAGTRELVIPRTPYIAAYVVMADRVRI
LRVLHGAQMWPDELDDK

Nucleotide

• Length: 294 bp

>T127866 NZ_CP041239:30337-30630 [Ensifer mexicanus]
ATGAGGCTTGTCTGGGCACGGTACGCCCTTGACGATCGGGACACCATCTTCAGTCGCATTGAAAGCGAGAACCCGAAGGC
GGCAATTCATGTCGACGAGGAGATCGTCCGCGCAGCACGTCGCCTTCTCGACTTTCCCGAAAGTGGTCGGCCTGGCCGGA
TTGCCGGAACGCGCGAGCTTGTCATCCCGCGCACGCCTTACATTGCTGCCTATGTGGTGATGGCGGATAGGGTTCGTATT
CTGCGTGTGTTGCATGGCGCTCAGATGTGGCCCGACGAGCTTGATGACAAGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11162.57 Da
• Isoelectric Point: 5.1554

>AT127866 WP_180941786.1 NZ_CP041239:30032-30340 [Ensifer mexicanus]
MVSNALVQTRIDADVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELVSHSEAYDAWFRAKVLQALEDTRSDVDDA
DADAHFRARRAAALRKAVVGDR

Nucleotide

• Length: 309 bp

>AT127866 NZ_CP041239:30032-30340 [Ensifer mexicanus]
ATGGTTTCAAATGCACTGGTCCAGACTCGGATAGATGCTGACGTCAAAGAGCGCGCCACGGCGGTCCTAGAAAACATGGG
CTTGACGGTCTCGGATGCCGTTCGAATCCTATTAACCCGGACGGCGAATGAAGGCGCCCTGCCGCTGGAACTTGTCAGCC
ATAGCGAGGCCTATGATGCCTGGTTCCGCGCCAAGGTATTGCAGGCGCTCGAGGACACCCGATCCGACGTCGATGATGCC
GATGCCGATGCGCACTTTCGCGCGCGCCGGGCGGCGGCCTTGCGCAAGGCCGTGGTGGGTGACCGATGA