Detailed information of TA system TA127863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2773340..2773908
• Replicon: chromosome
• Accession: NZ_CP041236
• Organism: Pseudomonas azotoformans strain P45A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FKZ69_RS13225
• Protein ID: WP_141606144.1
• Coordinates: 2773702..2773908 (-)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1C1W2P5
• Locus tag: FKZ69_RS13220
• Protein ID: WP_065939763.1
• Coordinates: 2773340..2773705 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKZ69_RS13195	2768829..2769635	+	807	WP_092489657.1	ammonia-dependent NAD(+) synthetase	-
FKZ69_RS13200	2769667..2769954	-	288	Protein_2557	ABC transporter substrate-binding protein	-
FKZ69_RS13205	2770076..2771431	+	1356	WP_092489658.1	MATE family efflux transporter	-
FKZ69_RS13210	2771415..2772002	-	588	WP_092489659.1	TetR/AcrR family transcriptional regulator	-
FKZ69_RS13215	2772144..2773316	+	1173	WP_027607493.1	MFS transporter	-
FKZ69_RS13220	2773340..2773705	-	366	WP_065939763.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FKZ69_RS13225	2773702..2773908	-	207	WP_141606144.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-61)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7800.08 Da
• Isoelectric Point: 9.7806

>T127863 WP_141606144.1 NZ_CP041236:c2773908-2773702 [Pseudomonas azotoformans]
MAFTWPELVTLLRRLGYTQIEGAGSRVKFDIGDPSAMITLHKPHPGNELKHYIRRQIIEQLKSGELIQ

Nucleotide

• Length: 207 bp

>T127863 NZ_CP041236:c2773908-2773702 [Pseudomonas azotoformans]
ATGGCATTTACCTGGCCAGAGCTCGTCACGCTGCTGCGTCGTCTCGGCTACACACAGATTGAGGGGGCTGGAAGCCGGGT
CAAGTTCGACATCGGCGATCCGAGCGCAATGATCACCTTGCACAAGCCTCATCCCGGCAACGAACTGAAACACTACATTC
GGCGCCAGATCATCGAACAATTGAAATCAGGAGAACTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13241.00 Da
• Isoelectric Point: 6.6276

>AT127863 WP_065939763.1 NZ_CP041236:c2773705-2773340 [Pseudomonas azotoformans]
VNNQLKHNGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFREAVDDYLATCQALGQTPEKPCKGSFNVRVGH
DLHLAAALEATRKKVTLNDLTRQALNEFLQHHRGDSCPVTG

Nucleotide

• Length: 366 bp

>AT127863 NZ_CP041236:c2773705-2773340 [Pseudomonas azotoformans]
GTGAACAACCAACTGAAACACAATGGCTACATCGGCTCTATCGAAGCCAGCCTCGAAGACAATTGCCTGTTCGGCAAGAT
CCTGTTCATCAAGGCACTGGTGAGTTACGAAGGCAAAACCGTCGCGGAACTGGACGCCGCGTTTCGCGAGGCGGTGGACG
ACTACCTCGCTACCTGCCAGGCCCTTGGGCAAACACCGGAGAAGCCGTGCAAAGGCTCTTTCAACGTGCGGGTCGGCCAC
GACCTTCATCTTGCCGCCGCCCTTGAAGCAACACGTAAAAAGGTGACCCTGAATGACCTGACGCGCCAGGCGCTGAACGA
ATTCCTGCAACACCACCGAGGGGACTCCTGCCCCGTGACTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1C1W2P5