Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2073530..2074134 Replicon chromosome
Accession NZ_CP041236
Organism Pseudomonas azotoformans strain P45A

Toxin (Protein)


Gene name hicA Uniprot ID A0A1X7G288
Locus tag FKZ69_RS09975 Protein ID WP_027607034.1
Coordinates 2073530..2073718 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1X7G3E9
Locus tag FKZ69_RS09980 Protein ID WP_027607035.1
Coordinates 2073730..2074134 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKZ69_RS09950 2068716..2069900 + 1185 WP_092489867.1 c-type cytochrome biogenesis protein CcmI -
FKZ69_RS09955 2069917..2070312 + 396 WP_092489866.1 hypothetical protein -
FKZ69_RS09965 2070852..2071424 + 573 WP_092489864.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
FKZ69_RS09970 2071791..2073287 + 1497 WP_141605866.1 M10 family metallopeptidase C-terminal domain-containing protein -
FKZ69_RS09975 2073530..2073718 + 189 WP_027607034.1 type II toxin-antitoxin system HicA family toxin Toxin
FKZ69_RS09980 2073730..2074134 + 405 WP_027607035.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FKZ69_RS09985 2074131..2075327 - 1197 WP_092489862.1 MFS transporter -
FKZ69_RS09990 2075469..2076077 + 609 WP_141605867.1 hypothetical protein -
FKZ69_RS09995 2076133..2077137 - 1005 WP_027607038.1 sulfate ABC transporter substrate-binding protein -
FKZ69_RS10000 2077234..2078058 - 825 WP_141605868.1 ion transporter -
FKZ69_RS10005 2078090..2078965 - 876 WP_141605869.1 urea transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7105.36 Da        Isoelectric Point: 11.5760

>T127862 WP_027607034.1 NZ_CP041236:2073530-2073718 [Pseudomonas azotoformans]
VQGRLLIKALQEAGWVLDRVTGSHPIFTHRYNPYTIPVPHPKKDLPLGTIRSIRKRAGLFQF

Download         Length: 189 bp

>T127862 NZ_CP041236:2073530-2073718 [Pseudomonas azotoformans]
GTGCAAGGCAGGCTATTGATCAAAGCGCTGCAAGAGGCGGGTTGGGTGCTGGATCGGGTCACGGGCAGTCATCCTATTTT
TACCCACCGCTATAACCCGTACACGATCCCGGTGCCGCATCCGAAGAAGGATCTGCCGCTGGGCACCATCAGAAGCATCA
GGAAACGTGCCGGGCTGTTTCAGTTTTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14743.74 Da        Isoelectric Point: 4.7465

>AT127862 WP_027607035.1 NZ_CP041236:2073730-2074134 [Pseudomonas azotoformans]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHILLQEIAAEGGVIPMPRSVGTHHTHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Download         Length: 405 bp

>AT127862 NZ_CP041236:2073730-2074134 [Pseudomonas azotoformans]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCAGGCGCAGT
CACCGCCGGGGACAGTTTCGAAGAGGCCTACAACGCCGCGGTGGAAGTCGCGCACATCCTGCTGCAGGAGATCGCCGCCG
AGGGCGGGGTGATTCCGATGCCAAGGTCGGTGGGCACTCATCACACCCATGAAGACTATGCCGGGATGGGCTGGGGCATG
CTGGAACTGGACATCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGCTATGTCATCCAGCGAAT
TGACCGGTATGTGCGCGAGCACAAGGTCAAAAGCCGCTCGTCATTTCTGGCGGATGCGGCTTTGGAGAAGTTGGTGCGTT
CCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X7G288

Antitoxin

Source ID Structure
AlphaFold DB A0A1X7G3E9

References