Detailed information of TA system TA127861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1677155..1677770
• Replicon: chromosome
• Accession: NZ_CP041236
• Organism: Pseudomonas azotoformans strain P45A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I4KFU3
• Locus tag: FKZ69_RS07905
• Protein ID: WP_003189411.1
• Coordinates: 1677588..1677770 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FKZ69_RS07900
• Protein ID: WP_141605683.1
• Coordinates: 1677155..1677565 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKZ69_RS07855	1672326..1672610	-	285	WP_141605678.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FKZ69_RS07860	1672600..1672839	-	240	WP_065928241.1	ribbon-helix-helix protein, CopG family	-
FKZ69_RS07865	1672940..1673350	+	411	WP_141605679.1	fosfomycin resistance glutathione transferase	-
FKZ69_RS07870	1673405..1674046	+	642	WP_027604357.1	LysE family translocator	-
FKZ69_RS07875	1674165..1674692	+	528	WP_027604356.1	DUF4142 domain-containing protein	-
FKZ69_RS07880	1674747..1675172	-	426	WP_053136892.1	low affinity iron permease family protein	-
FKZ69_RS07885	1675348..1676130	+	783	WP_141605680.1	GNAT family N-acetyltransferase	-
FKZ69_RS07890	1676131..1676631	-	501	WP_141605681.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
FKZ69_RS07895	1676725..1677066	-	342	WP_141605682.1	hypothetical protein	-
FKZ69_RS07900	1677155..1677565	-	411	WP_141605683.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FKZ69_RS07905	1677588..1677770	-	183	WP_003189411.1	type II toxin-antitoxin system HicA family toxin	Toxin
FKZ69_RS07910	1677935..1678831	-	897	WP_092487845.1	LysR family transcriptional regulator	-
FKZ69_RS07915	1678927..1680090	+	1164	WP_092487848.1	MFS transporter	-
FKZ69_RS07920	1680087..1681277	+	1191	WP_141605684.1	MFS transporter	-
FKZ69_RS07925	1681555..1681860	-	306	WP_027604346.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6804.01 Da
• Isoelectric Point: 10.7532

>T127861 WP_003189411.1 NZ_CP041236:c1677770-1677588 [Pseudomonas azotoformans]
VDSRYLIGHIVADGWYLVRIRGSHHHFKHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T127861 NZ_CP041236:c1677770-1677588 [Pseudomonas azotoformans]
GTGGATAGCCGATACTTGATAGGTCACATCGTCGCAGACGGCTGGTACCTGGTGCGCATCAGAGGCAGTCATCACCACTT
CAAGCACCCGACGAAGCCTGGCTTGGTGACGATCCCCCATCCAAAGAAAGACCTGTTGGACAAGACCGCCAAGAGCATTT
TGAAACAAGCTCTGTTGAGTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14837.77 Da
• Isoelectric Point: 4.7324

>AT127861 WP_141605683.1 NZ_CP041236:c1677565-1677155 [Pseudomonas azotoformans]
MLYPIAISTGDHDHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTVHAANPQYAGCTWAV
VDIDITKYLGKAQKLNITLPGYLLHRIDEYVLHHPEEKSRSGFLASAALKVLQQDR

Nucleotide

• Length: 411 bp

>AT127861 NZ_CP041236:c1677565-1677155 [Pseudomonas azotoformans]
ATGCTGTACCCCATCGCAATTTCAACCGGTGACCACGATCACGCCTGGGGCGTGGAAGTCCCGGATATTCCCGGTTGTTA
CTCTGCCGGTGACGACCTGGACGACGCCATGGCCATGGCGCGTGAAGCCATCGAAGGGCACTTCGAAATCCTCGCGGAAG
ACGGCGCGCCGATCCCGAGCGCCCAGAAGGTCACCGTGCATGCCGCCAACCCTCAGTACGCCGGCTGCACTTGGGCGGTT
GTAGACATCGATATCACCAAGTACCTGGGCAAGGCGCAAAAACTCAACATCACATTGCCGGGCTACCTGCTTCACCGCAT
CGATGAATATGTACTGCACCACCCGGAGGAAAAAAGCCGCTCGGGCTTCCTCGCGTCGGCGGCGCTCAAGGTGCTGCAAC
AGGATCGGTAG

Structures

Toxin

• AlphaFold DB: A0A1X7DFI9