Detailed information of TA system TA127856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3050..3638
• Replicon: plasmid pAhaeAN54c
• Accession: NZ_CP041226
• Organism: Acinetobacter haemolyticus strain AN54

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: AhaeAN54_RS17785
• Protein ID: WP_118901240.1
• Coordinates: 3050..3337 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: AhaeAN54_RS17790
• Protein ID: WP_118901243.1
• Coordinates: 3324..3638 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AhaeAN54_RS17765	1..575	-	575	WP_161595264.1	MobA/MobL family protein	-
AhaeAN54_RS17770	880..1638	+	759	WP_118901233.1	hypothetical protein	-
AhaeAN54_RS17775	2504..2821	+	318	WP_005065053.1	hypothetical protein	-
AhaeAN54_RS17785	3050..3337	+	288	WP_118901240.1	BrnT family toxin	Toxin
AhaeAN54_RS17790	3324..3638	+	315	WP_118901243.1	BrnA antitoxin family protein	Antitoxin
AhaeAN54_RS17795	3983..5290	-	1308	WP_086374879.1	NAD(P)/FAD-dependent oxidoreductase	-
AhaeAN54_RS17800	5307..6140	-	834	WP_086374880.1	S-formylglutathione hydrolase	-
AhaeAN54_RS17810	6614..7012	-	399	WP_086374882.1	VOC family protein	-
AhaeAN54_RS17815	7065..8174	-	1110	WP_086374883.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
AhaeAN54_RS17820	8196..8471	-	276	WP_031983787.1	metal/formaldehyde-sensitive transcriptional repressor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11418	11418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11335.67 Da
• Isoelectric Point: 4.9644

>T127856 WP_118901240.1 NZ_CP041226:3050-3337 [Acinetobacter haemolyticus]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHD

Nucleotide

• Length: 288 bp

>T127856 NZ_CP041226:3050-3337 [Acinetobacter haemolyticus]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATGTCTCTTTCGAAACTGCAAG
CCTTGTTTTTGATGATCCATTAAGGATCTCAATCCAAGACAGGCATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTCACCAAAGCGGAGCGAGATAGATATGAGCATGATTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12284.95 Da
• Isoelectric Point: 8.4842

>AT127856 WP_118901243.1 NZ_CP041226:3324-3638 [Acinetobacter haemolyticus]
MSMIRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT127856 NZ_CP041226:3324-3638 [Acinetobacter haemolyticus]
ATGAGCATGATTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ATAATCAATTCTATCGCCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGCTATCAAACACGAATGAATGAAATTTTGCGGGATGCTATGCTCAAAGAATTAAAAAATCATCAGTAA