Detailed information of TA system TA127854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2013011..2014647
• Replicon: chromosome
• Accession: NZ_CP041224
• Organism: Acinetobacter haemolyticus strain AN54

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A5P1UU42
• Locus tag: AhaeAN54_RS09875
• Protein ID: WP_005090545.1
• Coordinates: 2013011..2014342 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: AhaeAN54_RS09880
• Protein ID: WP_005090546.1
• Coordinates: 2014339..2014647 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AhaeAN54_RS09860	2008630..2010822	+	2193	WP_115736425.1	TonB-dependent siderophore receptor	-
AhaeAN54_RS09865	2010869..2012011	+	1143	Protein_1898	WG repeat-containing protein	-
AhaeAN54_RS09870	2012075..2012773	-	699	WP_005090543.1	DUF1826 domain-containing protein	-
AhaeAN54_RS09875	2013011..2014342	+	1332	WP_005090545.1	HipA domain-containing protein	Toxin
AhaeAN54_RS09880	2014339..2014647	+	309	WP_005090546.1	helix-turn-helix domain-containing protein	Antitoxin
AhaeAN54_RS09885	2014689..2015363	+	675	WP_005090547.1	DUF4145 domain-containing protein	-
AhaeAN54_RS09890	2015631..2016665	-	1035	WP_171265829.1	AraC family transcriptional regulator	-
AhaeAN54_RS09895	2016797..2017498	+	702	WP_005080784.1	NAD(P)H-dependent oxidoreductase	-
AhaeAN54_RS09900	2017571..2018320	-	750	WP_118902342.1	iron-containing redox enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (167-387)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-83)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 50765.55 Da
• Isoelectric Point: 4.8292

>T127854 WP_005090545.1 NZ_CP041224:2013011-2014342 [Acinetobacter haemolyticus]
MQKLTIQALLDQNWLDIAELKLLEPKLGSASASELVYELEYAIQNLDKRDEHACSLSLPVQILINHESSSWFGFLDDIVP
SGAARRYWVNFLGLQRLTYAEQDSILLEKGGIAPVGNLRIKEALPPINPDSTLHLRYFSQNDVADRNVDFLEYAQQMGAI
SGGATGAGGEAPKLLIRATKDQNIWIDTYQQNFDQPDQHYLVKFPRNNRSEIDCNILRAEYYYYQELKELGFNTIETTEM
KLIEGEKYPSLWLPRFDVEWRNQCWQRHGLESVYSVLNKASGSHLNHFEVIDNLCDLLTVIDSNFDSTQFVCEWLQRDLL
NIIFGNSDNHGRNTSFLKKSGKISLAPIYDFAPMKADPEVVTRSTTWGSPYEEGGEYRWEQITQKLAHLCDPNLSMKTLK
SLANNLVGLKQRLIDKGVPKQIMEMPALSFDYVEAKLERWELL

Nucleotide

• Length: 1332 bp

>T127854 NZ_CP041224:2013011-2014342 [Acinetobacter haemolyticus]
ATGCAAAAATTAACAATACAGGCTTTACTCGACCAGAATTGGTTAGATATCGCTGAACTTAAACTATTAGAACCTAAGCT
GGGCTCAGCAAGCGCCAGTGAATTGGTCTACGAGTTGGAATACGCAATCCAAAATCTGGACAAGCGAGATGAACATGCCT
GTAGTCTCTCACTTCCTGTACAAATACTTATAAACCATGAATCTAGCTCATGGTTTGGTTTTTTGGATGATATTGTTCCT
TCAGGCGCAGCGCGCCGTTATTGGGTCAATTTTTTAGGCTTACAACGACTCACTTATGCTGAGCAAGATAGTATTTTATT
GGAAAAGGGTGGAATTGCGCCCGTAGGAAACTTGCGAATTAAAGAAGCTCTTCCGCCTATAAATCCTGACTCTACTTTGC
ATTTACGTTATTTTAGTCAAAATGATGTTGCTGATCGAAATGTTGATTTTCTTGAGTATGCACAGCAAATGGGCGCGATT
AGTGGTGGTGCAACAGGAGCGGGTGGTGAAGCGCCCAAGTTACTGATTCGCGCGACAAAAGATCAAAATATTTGGATTGA
CACCTATCAACAAAACTTTGATCAACCTGATCAACACTATTTAGTTAAATTTCCTCGAAATAATCGTAGTGAGATCGACT
GCAATATTCTCAGAGCGGAATACTATTACTATCAAGAACTAAAAGAATTAGGTTTCAATACCATTGAAACAACAGAAATG
AAGCTGATCGAAGGTGAAAAATATCCATCATTATGGTTGCCAAGATTTGATGTGGAATGGCGCAATCAATGTTGGCAACG
TCATGGTCTAGAATCTGTTTATTCAGTATTAAATAAGGCTTCAGGAAGTCATCTTAATCATTTTGAAGTAATAGACAACC
TATGTGATTTACTGACAGTGATTGATTCAAATTTTGATTCCACACAATTCGTTTGTGAATGGTTACAAAGAGACTTGCTC
AATATTATTTTTGGCAATTCTGATAATCACGGGCGAAATACCTCATTTCTAAAAAAATCTGGAAAAATTTCGCTTGCTCC
AATTTATGATTTTGCACCAATGAAAGCTGATCCAGAAGTCGTGACAAGATCAACGACATGGGGAAGTCCTTATGAAGAAG
GAGGAGAGTATCGTTGGGAGCAAATTACCCAGAAACTTGCACATTTATGCGATCCAAATTTGTCTATGAAGACATTAAAA
TCATTAGCAAACAATCTTGTTGGGCTAAAACAACGATTAATTGATAAAGGTGTCCCAAAACAGATTATGGAGATGCCAGC
ACTTTCATTCGATTATGTTGAAGCTAAATTAGAAAGATGGGAACTATTATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11716.80 Da
• Isoelectric Point: 10.1562

>AT127854 WP_005090546.1 NZ_CP041224:2014339-2014647 [Acinetobacter haemolyticus]
MNIKNQPQDRQKVLIDLYMQYLLNEITLGQLLAYLRKNILGMSQEQYANLVGVSRRTLTDIEQDKGKLTQSVLDKVFKPL
GLKAGLVPTHEHIVRKIIKLSE

Nucleotide

• Length: 309 bp

>AT127854 NZ_CP041224:2014339-2014647 [Acinetobacter haemolyticus]
ATGAATATAAAAAATCAGCCTCAAGATCGCCAGAAAGTTCTTATTGATCTATATATGCAGTACTTATTGAATGAAATTAC
CTTAGGGCAATTATTGGCGTATTTGCGAAAAAATATACTTGGCATGTCTCAGGAACAATACGCAAATTTGGTCGGTGTCA
GTCGCAGAACATTAACGGACATAGAACAAGACAAAGGAAAACTCACGCAATCTGTTTTAGATAAAGTATTTAAACCACTG
GGATTGAAAGCAGGACTTGTACCGACTCATGAGCATATTGTGAGGAAGATTATTAAATTATCAGAGTGA

Structures

Toxin

• AlphaFold DB: A0A5P1UU42