Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 4078292..4078927 Replicon chromosome
Accession NZ_CP041217
Organism Saccharibacillus brassicae strain ATSA2

Toxin (Protein)


Gene name mazF Uniprot ID A0A4Y6UXB1
Locus tag FFV09_RS16880 Protein ID WP_018975909.1
Coordinates 4078577..4078927 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A254NWT8
Locus tag FFV09_RS16875 Protein ID WP_018975910.1
Coordinates 4078292..4078573 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFV09_RS16855 4073607..4074446 - 840 WP_141450511.1 proline/glycine betaine ABC transporter permease -
FFV09_RS16860 4074463..4075668 - 1206 WP_141448912.1 glycine betaine/L-proline ABC transporter ATP-binding protein -
FFV09_RS16865 4076109..4076675 + 567 WP_141448913.1 GbsR/MarR family transcriptional regulator -
FFV09_RS16870 4076912..4078120 + 1209 WP_170315062.1 alanine racemase -
FFV09_RS16875 4078292..4078573 + 282 WP_018975910.1 ribbon-helix-helix protein, CopG family Antitoxin
FFV09_RS16880 4078577..4078927 + 351 WP_018975909.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FFV09_RS16885 4079412..4080092 + 681 WP_141448915.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
FFV09_RS16890 4080097..4081056 + 960 WP_141448916.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
FFV09_RS16895 4081327..4082262 + 936 WP_141448917.1 RNA methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12912.89 Da        Isoelectric Point: 6.4717

>T127847 WP_018975909.1 NZ_CP041217:4078577-4078927 [Saccharibacillus brassicae]
MLVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTHGFDRDSVVLLEQI
RTIDKQRLTDRITRLGEETMKRVDESLQISLSLVDF

Download         Length: 351 bp

>T127847 NZ_CP041217:4078577-4078927 [Saccharibacillus brassicae]
TTGCTTGTAAAACGCGGTGACGTATTTTTTGCGGATCTCTCGCCAGTGGTGGGATCCGAGCAGGGCGGGGTGCGTCCGGT
TCTGGTGATCCAGAACGATATCGGCAACCGCTTTAGCCCTACGGTCATCGTCGCGGCGATTACCGCCCAGATTCAAAAAG
CCAAGCTGCCGACGCACGTGGAGATCGATGCCAAGACGCACGGATTCGATCGCGATTCCGTCGTCCTGCTCGAACAGATT
CGAACGATCGACAAGCAGCGCCTGACCGACCGGATTACGAGACTCGGCGAAGAAACGATGAAGCGGGTCGACGAGTCTTT
GCAAATCAGCTTGAGCTTGGTTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10633.27 Da        Isoelectric Point: 6.2191

>AT127847 WP_018975910.1 NZ_CP041217:4078292-4078573 [Saccharibacillus brassicae]
VANLQSTKRVMISLPDHLLQEVDGIAALENSNRSELIRLAMRQYLVDRKKRYIRESMQQGYMEMAHINLSMACEAFHAEE
DADITLVRLVSGV

Download         Length: 282 bp

>AT127847 NZ_CP041217:4078292-4078573 [Saccharibacillus brassicae]
GTGGCGAACTTGCAAAGCACCAAGAGAGTGATGATCAGCCTGCCTGATCATCTTCTGCAGGAAGTGGATGGGATCGCAGC
TTTGGAAAATTCCAATCGTAGTGAACTGATCAGGCTGGCAATGAGACAGTATTTGGTCGACCGGAAGAAGCGGTATATCC
GCGAGTCGATGCAGCAGGGTTACATGGAAATGGCCCACATCAATTTGTCGATGGCTTGTGAAGCGTTTCATGCGGAGGAA
GATGCGGACATCACTCTGGTTCGCTTAGTAAGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y6UXB1

Antitoxin

Source ID Structure
AlphaFold DB A0A254NWT8

References