Detailed information of TA system TA127845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 2142..2719
• Replicon: plasmid pCFSAN074382
• Accession: NZ_CP041214
• Organism: Listeria monocytogenes strain LMP18-H8393

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: DVH16_RS15505
• Protein ID: WP_003728465.1
• Coordinates: 2378..2719 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: DVH16_RS15500
• Protein ID: WP_003728464.1
• Coordinates: 2142..2384 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVH16_RS15480	315..692	-	378	WP_003769263.1	DUF3850 domain-containing protein	-
DVH16_RS15485	711..1373	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
DVH16_RS15490	1373..1699	-	327	WP_003728462.1	hypothetical protein	-
DVH16_RS15495	1830..2081	+	252	WP_003728463.1	hypothetical protein	-
DVH16_RS15500	2142..2384	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DVH16_RS15505	2378..2719	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DVH16_RS15510	2912..5047	-	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
DVH16_RS15515	5047..5406	-	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
DVH16_RS15520	5686..6240	+	555	WP_003728468.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57553	57553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-105)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 14629.86 Da
• Isoelectric Point: 12.8531

>T127845 WP_003728465.1 NZ_CP041214:2378-2719 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 342 bp

>T127845 NZ_CP041214:2378-2719 [Listeria monocytogenes]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 10400.12 Da
• Isoelectric Point: 12.7030

>AT127845 WP_003728464.1 NZ_CP041214:2142-2384 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
K

Nucleotide

• Length: 243 bp

>AT127845 NZ_CP041214:2142-2384 [Listeria monocytogenes]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5