Detailed information of TA system TA127840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3713918..3714592
• Replicon: chromosome
• Accession: NZ_CP041207
• Organism: Mycobacterium tuberculosis strain MT-0080

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: FKO20_RS18185
• Protein ID: WP_003417282.1
• Coordinates: 3713918..3714346 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: FKO20_RS18190
• Protein ID: WP_003417286.1
• Coordinates: 3714350..3714592 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKO20_RS18160	3709740..3710141	-	402	WP_003417264.1	cytidine deaminase	-
FKO20_RS18165	3710282..3710716	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
FKO20_RS18170	3710713..3711147	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
FKO20_RS18175	3711276..3713048	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
FKO20_RS18180	3713048..3713839	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
FKO20_RS18185	3713918..3714346	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
FKO20_RS18190	3714350..3714592	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
FKO20_RS18195	3714714..3715328	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
FKO20_RS18200	3715325..3715990	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
FKO20_RS18205	3715991..3716524	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
FKO20_RS18210	3716521..3716895	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
FKO20_RS18215	3716992..3718056	-	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T127840 WP_003417282.1 NZ_CP041207:c3714346-3713918 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T127840 NZ_CP041207:c3714346-3713918 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT127840 WP_003417286.1 NZ_CP041207:c3714592-3714350 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT127840 NZ_CP041207:c3714592-3714350 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59