Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3079293..3079980 Replicon chromosome
Accession NZ_CP041207
Organism Mycobacterium tuberculosis strain MT-0080

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag FKO20_RS15110 Protein ID WP_003414064.1
Coordinates 3079585..3079980 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FKO20_RS15105 Protein ID WP_003414061.1
Coordinates 3079293..3079559 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKO20_RS15080 3074932..3075834 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
FKO20_RS15085 3075903..3076655 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FKO20_RS15090 3076899..3077174 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
FKO20_RS15095 3077171..3078793 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
FKO20_RS15100 3078880..3079296 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
FKO20_RS15105 3079293..3079559 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FKO20_RS15110 3079585..3079980 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
FKO20_RS15115 3079977..3080246 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FKO20_RS15120 3080256..3081350 - 1095 WP_003900565.1 restriction endonuclease subunit S -
FKO20_RS15125 3081347..3081766 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FKO20_RS15130 3081840..3082319 - 480 WP_003414073.1 dihydrofolate reductase -
FKO20_RS15135 3082390..3083190 - 801 WP_003911953.1 thymidylate synthase -
FKO20_RS15140 3083346..3084083 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T127833 WP_003414064.1 NZ_CP041207:c3079980-3079585 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T127833 NZ_CP041207:c3079980-3079585 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT127833 WP_003414061.1 NZ_CP041207:c3079559-3079293 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT127833 NZ_CP041207:c3079559-3079293 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References