Detailed information of TA system TA127828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2878571..2879202
• Replicon: chromosome
• Accession: NZ_CP041207
• Organism: Mycobacterium tuberculosis strain MT-0080

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FKO20_RS13960
• Protein ID: WP_003413174.1
• Coordinates: 2878825..2879202 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FKO20_RS13955
• Protein ID: WP_145830704.1
• Coordinates: 2878571..2878828 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKO20_RS13915	2873829..2874143	+	315	WP_009937839.1	hypothetical protein	-
FKO20_RS13920	2874439..2875650	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FKO20_RS13930	2875777..2876436	+	660	WP_031651883.1	LppA family lipoprotein	-
FKO20_RS13935	2876433..2877092	+	660	WP_003900847.1	hypothetical protein	-
FKO20_RS13940	2877089..2877751	+	663	WP_003900848.1	hypothetical protein	-
FKO20_RS13945	2877748..2878026	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FKO20_RS13950	2878119..2878532	+	414	WP_003413164.1	PIN domain nuclease	-
FKO20_RS13955	2878571..2878828	+	258	WP_145830704.1	ribbon-helix-helix domain-containing protein	Antitoxin
FKO20_RS13960	2878825..2879202	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FKO20_RS13965	2879218..2879592	-	375	WP_003413177.1	hypothetical protein	-
FKO20_RS13970	2879692..2880087	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FKO20_RS13975	2880084..2880329	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FKO20_RS13985	2880740..2881159	-	420	WP_003413190.1	A24 family peptidase	-
FKO20_RS13990	2881171..2881980	-	810	WP_003413193.1	shikimate dehydrogenase	-
FKO20_RS13995	2881977..2883230	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FKO20_RS14000	2883223..2883735	-	513	WP_145830705.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T127828 WP_003413174.1 NZ_CP041207:2878825-2879202 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T127828 NZ_CP041207:2878825-2879202 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9423.61 Da
• Isoelectric Point: 10.9854

>AT127828 WP_145830704.1 NZ_CP041207:2878571-2878828 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSRRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT127828 NZ_CP041207:2878571-2878828 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGCGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28