Detailed information of TA system TA127785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366182..366753
• Replicon: chromosome
• Accession: NZ_CP041207
• Organism: Mycobacterium tuberculosis strain MT-0080

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: FKO20_RS01625
• Protein ID: WP_003401560.1
• Coordinates: 366182..366484 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: FKO20_RS01630
• Protein ID: WP_003401563.1
• Coordinates: 366532..366753 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKO20_RS01605	361651..362454	-	804	WP_003401540.1	hypothetical protein	-
FKO20_RS01610	362464..363861	-	1398	WP_003401544.1	sulfatase	-
FKO20_RS01615	364040..365815	+	1776	WP_010886074.1	PE family protein	-
FKO20_RS01620	365958..366185	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
FKO20_RS01625	366182..366484	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
FKO20_RS01630	366532..366753	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
FKO20_RS01635	366750..367175	+	426	WP_003401566.1	PIN domain nuclease	-
FKO20_RS01640	367311..367943	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
FKO20_RS01645	367940..368848	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T127785 WP_003401560.1 NZ_CP041207:366182-366484 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T127785 NZ_CP041207:366182-366484 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT127785 WP_003401563.1 NZ_CP041207:366532-366753 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT127785 NZ_CP041207:366532-366753 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48