Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 559623..560259 Replicon chromosome
Accession NZ_CP041154
Organism Bacillus licheniformis strain CSL2

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag BLCSL_RS02785 Protein ID WP_003179128.1
Coordinates 559909..560259 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag BLCSL_RS02780 Protein ID WP_006638778.1
Coordinates 559623..559904 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLCSL_RS02760 554738..556219 + 1482 WP_009330132.1 PH domain-containing protein -
BLCSL_RS02765 556216..556815 - 600 WP_003179118.1 rhomboid family intramembrane serine protease -
BLCSL_RS02770 557158..558117 + 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
BLCSL_RS02775 558342..559511 + 1170 WP_025807384.1 alanine racemase -
BLCSL_RS02780 559623..559904 + 282 WP_006638778.1 hypothetical protein Antitoxin
BLCSL_RS02785 559909..560259 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BLCSL_RS02790 560377..561204 + 828 WP_003179130.1 RsbT co-antagonist protein RsbRA -
BLCSL_RS02795 561208..561573 + 366 WP_003179132.1 STAS domain-containing protein -
BLCSL_RS02800 561576..561977 + 402 WP_003179135.1 anti-sigma regulatory factor -
BLCSL_RS02805 561988..562995 + 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
BLCSL_RS02810 563054..563380 + 327 WP_003179140.1 anti-sigma factor antagonist -
BLCSL_RS02815 563380..563865 + 486 WP_003179142.1 anti-sigma B factor RsbW -
BLCSL_RS02820 563831..564622 + 792 WP_003179144.1 RNA polymerase sigma factor SigB -
BLCSL_RS02825 564619..565218 + 600 WP_003179145.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T127745 WP_003179128.1 NZ_CP041154:559909-560259 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T127745 NZ_CP041154:559909-560259 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT127745 WP_006638778.1 NZ_CP041154:559623-559904 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT127745 NZ_CP041154:559623-559904 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References