Detailed information of TA system TA127744

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 3176803..3177460
• Replicon: chromosome
• Accession: NZ_CP041153
• Organism: Shewanella marisflavi strain ECSMB14101

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: -
• Locus tag: FGA12_RS14090
• Protein ID: WP_033539775.1
• Coordinates: 3176803..3177240 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A0A4Y6J699
• Locus tag: FGA12_RS14095
• Protein ID: WP_033539774.1
• Coordinates: 3177221..3177460 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGA12_RS14065	3172279..3172755	-	477	WP_033539780.1	SoxR reducing system RseC family protein	-
FGA12_RS14070	3172763..3173695	-	933	WP_033539779.1	MucB/RseB C-terminal domain-containing protein	-
FGA12_RS14075	3173760..3174365	-	606	WP_033539778.1	anti-sigma factor	-
FGA12_RS14080	3174405..3174983	-	579	WP_033539777.1	RNA polymerase sigma factor RpoE	-
FGA12_RS14085	3175183..3176796	+	1614	WP_033539776.1	L-aspartate oxidase	-
FGA12_RS14090	3176803..3177240	-	438	WP_033539775.1	hypothetical protein	Toxin
FGA12_RS14095	3177221..3177460	-	240	WP_033539774.1	succinate dehydrogenase assembly factor 2	Antitoxin
FGA12_RS14100	3177538..3178446	-	909	WP_033539773.1	transcriptional activator NhaR	-
FGA12_RS14105	3178469..3178852	-	384	WP_033539772.1	hypothetical protein	-
FGA12_RS14110	3178849..3180024	-	1176	WP_033539771.1	Na+/H+ antiporter NhaA	-
FGA12_RS14115	3180333..3181184	-	852	WP_033539770.1	thymidylate synthase	-
FGA12_RS14120	3181261..3182055	-	795	WP_033539769.1	sulfite exporter TauE/SafE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-143)

Antitoxin

• Flavinator of succinate dehydrogenase (4-74)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 17035.04 Da
• Isoelectric Point: 8.9318

>T127744 WP_033539775.1 NZ_CP041153:c3177240-3176803 [Shewanella marisflavi]
VAGRRHKFHLVASFDQRLSLICFAAVCLSSFLVWPPISLLWFVIIKYALLSAVVGFFVYQFWILKHWSFGFWLDESGKIG
LDEKSQGAAQTENCVALYYRRVWVSPFAVLFWLCSDDNKRLVILWRDMFDDTGYRHLCRLLLRFG

Nucleotide

• Length: 438 bp

>T127744 NZ_CP041153:c3177240-3176803 [Shewanella marisflavi]
GTGGCCGGGCGGCGCCATAAGTTTCACCTTGTCGCTTCATTTGATCAGCGACTCTCGCTGATATGTTTTGCGGCCGTTTG
TCTAAGTTCATTCTTGGTCTGGCCGCCAATTTCCCTTCTCTGGTTTGTTATCATCAAATACGCCCTGCTATCGGCAGTGG
TCGGTTTCTTTGTTTATCAATTTTGGATACTCAAACACTGGTCATTTGGCTTCTGGCTGGATGAGTCCGGTAAGATTGGG
CTGGATGAAAAGTCGCAAGGCGCTGCGCAAACAGAAAACTGTGTGGCTCTGTATTATCGGCGGGTGTGGGTGAGTCCCTT
TGCCGTACTTTTTTGGCTTTGTAGTGATGACAATAAGCGTTTAGTGATTCTGTGGCGGGATATGTTTGACGACACAGGCT
ATCGGCACCTGTGTCGTCTATTGCTCAGATTTGGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9192.70 Da
• Isoelectric Point: 4.7627

>AT127744 WP_033539774.1 NZ_CP041153:c3177460-3177221 [Shewanella marisflavi]
MNIARVRWACRRGMLELDVLFQPFVETQYEHLSNDDKAVFVRLLECEDPELFAWFMGHEVCPDPLLADMVVKVRGRAAP

Nucleotide

• Length: 240 bp

>AT127744 NZ_CP041153:c3177460-3177221 [Shewanella marisflavi]
ATGAACATTGCCAGAGTGAGATGGGCGTGCCGACGCGGCATGTTAGAGCTTGATGTATTGTTTCAACCCTTTGTTGAAAC
GCAATATGAGCATCTTTCTAATGATGACAAAGCTGTTTTTGTTCGTTTGTTAGAGTGTGAAGATCCCGAGTTGTTTGCCT
GGTTTATGGGTCATGAAGTGTGTCCCGATCCACTGCTTGCCGATATGGTCGTAAAAGTTCGTGGCCGGGCGGCGCCATAA

Structures

Antitoxin

• AlphaFold DB: A0A4Y6J699