Detailed information of TA system TA127732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2808505..2809112
• Replicon: chromosome
• Accession: NZ_CP041146
• Organism: Nocardioides humi strain DCY24

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FIV44_RS13730
• Protein ID: WP_141004932.1
• Coordinates: 2808705..2809112 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FIV44_RS13725
• Protein ID: WP_141004931.1
• Coordinates: 2808505..2808717 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIV44_RS13715	2804007..2805608	-	1602	WP_141004929.1	helix-turn-helix transcriptional regulator	-
FIV44_RS13720	2805675..2808287	-	2613	WP_141004930.1	hypothetical protein	-
FIV44_RS13725	2808505..2808717	+	213	WP_141004931.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FIV44_RS13730	2808705..2809112	+	408	WP_141004932.1	PIN domain nuclease	Toxin
FIV44_RS13735	2809137..2810789	-	1653	WP_141004933.1	helix-turn-helix transcriptional regulator	-
FIV44_RS13740	2810910..2811515	+	606	WP_141004934.1	DUF3558 domain-containing protein	-
FIV44_RS13745	2811744..2812049	-	306	WP_141007872.1	DUF2470 domain-containing protein	-
FIV44_RS13750	2812072..2812764	-	693	WP_141004935.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-34)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15053.31 Da
• Isoelectric Point: 6.2221

>T127732 WP_141004932.1 NZ_CP041146:2808705-2809112 [Nocardioides humi]
MGRVTLVDTSVWIDFLRRPGHPGNEPLRELLRHEEAVTTPPIEMELLLGPTDELSLRRVEKAIVGVGTLPVQPDVDFHDA
AAIYRAVRRSGRTVRNSVDCLIAAIAIRHEVPLLHRDADFEAIAGVTELQHRSLL

Nucleotide

• Length: 408 bp

>T127732 NZ_CP041146:2808705-2809112 [Nocardioides humi]
ATGGGACGAGTGACACTGGTCGACACGTCGGTCTGGATCGACTTCCTCCGGCGCCCCGGGCATCCCGGCAATGAGCCATT
GCGCGAGCTGCTCCGGCACGAGGAAGCAGTGACGACCCCGCCGATCGAGATGGAGCTGCTCCTGGGTCCGACCGATGAGT
TGTCTCTTCGCCGTGTGGAGAAGGCCATCGTCGGTGTGGGCACCCTTCCGGTCCAGCCGGACGTCGACTTCCACGACGCG
GCCGCGATCTATCGTGCGGTACGTCGCAGTGGACGCACCGTGCGCAACAGCGTCGACTGCCTGATCGCCGCCATCGCGAT
CCGCCACGAAGTGCCGCTGCTCCACAGGGACGCCGACTTCGAGGCGATCGCCGGCGTGACCGAGCTGCAGCACCGGTCCC
TGCTCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7877.93 Da
• Isoelectric Point: 4.7571

>AT127732 WP_141004931.1 NZ_CP041146:2808505-2808717 [Nocardioides humi]
MTRTNIDIDDELIARVMKKYGLRTKREAVDLALRRAAGPKVTPEEILALEGTGWDGDLGQIRNNPSPWDE

Nucleotide

• Length: 213 bp

>AT127732 NZ_CP041146:2808505-2808717 [Nocardioides humi]
ATGACACGCACCAATATCGACATCGACGACGAGCTGATCGCCCGTGTCATGAAGAAGTACGGCCTGCGGACCAAGCGGGA
GGCGGTCGACTTGGCGTTGCGCCGGGCGGCCGGTCCCAAGGTCACGCCGGAGGAGATCTTGGCGTTGGAGGGCACCGGCT
GGGACGGCGATCTCGGCCAGATCCGCAACAACCCCTCGCCATGGGACGAGTGA