Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 490878..491515 Replicon chromosome
Accession NZ_CP041145
Organism Bacillus velezensis strain At1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag D073_RS02500 Protein ID WP_003156187.1
Coordinates 491165..491515 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag D073_RS02495 Protein ID WP_003156188.1
Coordinates 490878..491159 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D073_RS02475 487243..487842 - 600 WP_020955355.1 rhomboid family intramembrane serine protease -
D073_RS02480 487935..488300 + 366 WP_007410229.1 holo-ACP synthase -
D073_RS02485 488465..489472 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
D073_RS02490 489589..490758 + 1170 WP_020955356.1 alanine racemase -
D073_RS02495 490878..491159 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
D073_RS02500 491165..491515 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D073_RS02505 491633..492454 + 822 WP_003156182.1 STAS domain-containing protein -
D073_RS02510 492459..492824 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
D073_RS02515 492827..493228 + 402 WP_003156178.1 anti-sigma regulatory factor -
D073_RS02520 493240..494247 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
D073_RS02525 494311..494640 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
D073_RS02530 494637..495119 + 483 WP_003156173.1 anti-sigma B factor RsbW -
D073_RS02535 495085..495873 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
D073_RS02540 495873..496475 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T127728 WP_003156187.1 NZ_CP041145:491165-491515 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T127728 NZ_CP041145:491165-491515 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT127728 WP_003156188.1 NZ_CP041145:490878-491159 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT127728 NZ_CP041145:490878-491159 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References