Detailed information of TA system TA127727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1221506..1222423
• Replicon: chromosome
• Accession: NZ_CP041144
• Organism: Bacillus velezensis strain UCMB5044

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: FIM06_RS06405
• Protein ID: WP_007407256.1
• Coordinates: 1221677..1222423 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: FIM06_RS06400
• Protein ID: WP_003154807.1
• Coordinates: 1221506..1221676 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIM06_RS06355	1216659..1217171	+	513	WP_105937829.1	Fis family transcriptional regulator	-
FIM06_RS06360	1217284..1217505	+	222	Protein_1169	terminase	-
FIM06_RS06365	1217894..1218361	+	468	WP_140957745.1	hypothetical protein	-
FIM06_RS06370	1218374..1218745	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
FIM06_RS06375	1218750..1218947	+	198	WP_105937827.1	XkdX family protein	-
FIM06_RS06380	1219004..1219765	+	762	WP_105937826.1	phage portal protein	-
FIM06_RS06385	1219817..1220080	+	264	WP_003154815.1	hemolysin XhlA family protein	-
FIM06_RS06390	1220094..1220357	+	264	WP_003154813.1	phage holin	-
FIM06_RS06395	1220371..1221249	+	879	WP_105937825.1	N-acetylmuramoyl-L-alanine amidase	-
FIM06_RS06400	1221506..1221676	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
FIM06_RS06405	1221677..1222423	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FIM06_RS06410	1222531..1223529	-	999	WP_044802872.1	inorganic phosphate transporter	-
FIM06_RS06415	1223542..1224159	-	618	WP_032874614.1	DUF47 domain-containing protein	-
FIM06_RS06420	1224445..1225761	-	1317	WP_045207179.1	amino acid permease	-
FIM06_RS06425	1226084..1227034	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1207583..1279772	72189

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T127727 WP_007407256.1 NZ_CP041144:c1222423-1221677 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T127727 NZ_CP041144:c1222423-1221677 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATAAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAATCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT127727 WP_003154807.1 NZ_CP041144:c1221676-1221506 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT127727 NZ_CP041144:c1221676-1221506 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14