Detailed information of TA system TA127725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1221523..1222440
• Replicon: chromosome
• Accession: NZ_CP041143
• Organism: Bacillus velezensis strain UCMB5007

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: D069_RS06410
• Protein ID: WP_007407256.1
• Coordinates: 1221694..1222440 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: D069_RS06405
• Protein ID: WP_003154807.1
• Coordinates: 1221523..1221693 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D069_RS06360	1216676..1217188	+	513	WP_105937829.1	Fis family transcriptional regulator	-
D069_RS06365	1217301..1217522	+	222	Protein_1169	terminase	-
D069_RS06370	1217911..1218378	+	468	WP_140957745.1	hypothetical protein	-
D069_RS06375	1218391..1218762	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
D069_RS06380	1218767..1218964	+	198	WP_105937827.1	XkdX family protein	-
D069_RS06385	1219021..1219782	+	762	WP_105937826.1	phage portal protein	-
D069_RS06390	1219834..1220097	+	264	WP_003154815.1	hemolysin XhlA family protein	-
D069_RS06395	1220111..1220374	+	264	WP_003154813.1	phage holin	-
D069_RS06400	1220388..1221266	+	879	WP_105937825.1	N-acetylmuramoyl-L-alanine amidase	-
D069_RS06405	1221523..1221693	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
D069_RS06410	1221694..1222440	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
D069_RS06415	1222548..1223546	-	999	WP_044802872.1	inorganic phosphate transporter	-
D069_RS06420	1223559..1224176	-	618	WP_032874614.1	DUF47 domain-containing protein	-
D069_RS06425	1224462..1225778	-	1317	WP_045207179.1	amino acid permease	-
D069_RS06430	1226101..1227051	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1207600..1279789	72189

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T127725 WP_007407256.1 NZ_CP041143:c1222440-1221694 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T127725 NZ_CP041143:c1222440-1221694 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATAAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAATCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT127725 WP_003154807.1 NZ_CP041143:c1221693-1221523 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT127725 NZ_CP041143:c1221693-1221523 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14