Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 482680..483317 Replicon chromosome
Accession NZ_CP041143
Organism Bacillus velezensis strain UCMB5007

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag D069_RS02485 Protein ID WP_003156187.1
Coordinates 482967..483317 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag D069_RS02480 Protein ID WP_003156188.1
Coordinates 482680..482961 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D069_RS02460 479045..479644 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
D069_RS02465 479737..480102 + 366 WP_105938067.1 holo-ACP synthase -
D069_RS02470 480267..481274 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
D069_RS02475 481391..482560 + 1170 WP_105938066.1 alanine racemase -
D069_RS02480 482680..482961 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
D069_RS02485 482967..483317 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D069_RS02490 483435..484256 + 822 WP_003156182.1 STAS domain-containing protein -
D069_RS02495 484261..484626 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
D069_RS02500 484629..485030 + 402 WP_003156178.1 anti-sigma regulatory factor -
D069_RS02505 485042..486049 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
D069_RS02510 486113..486442 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
D069_RS02515 486439..486921 + 483 WP_007609591.1 anti-sigma B factor RsbW -
D069_RS02520 486887..487675 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
D069_RS02525 487675..488277 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T127724 WP_003156187.1 NZ_CP041143:482967-483317 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T127724 NZ_CP041143:482967-483317 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT127724 WP_003156188.1 NZ_CP041143:482680-482961 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT127724 NZ_CP041143:482680-482961 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References