Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1066869..1067491 Replicon chromosome
Accession NZ_CP041134
Organism Serratia marcescens strain WVU-010

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag FG175_RS05045 Protein ID WP_004940313.1
Coordinates 1066869..1067072 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag FG175_RS05050 Protein ID WP_004940312.1
Coordinates 1067123..1067491 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FG175_RS05020 1062679..1063125 + 447 WP_141958128.1 type II secretion system protein M -
FG175_RS05025 1063161..1063976 + 816 WP_141958130.1 A24 family peptidase -
FG175_RS05030 1063973..1064335 - 363 WP_060423419.1 type II secretion system pilot lipoprotein GspS -
FG175_RS05035 1064588..1066015 + 1428 WP_060423422.1 N-acetylglucosamine-binding protein GbpA -
FG175_RS05040 1066068..1066586 + 519 WP_060423424.1 cytochrome b/b6 domain-containing protein -
FG175_RS05045 1066869..1067072 - 204 WP_004940313.1 hemolysin expression modulator Hha Toxin
FG175_RS05050 1067123..1067491 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
FG175_RS05055 1067650..1068003 - 354 WP_060423427.1 hypothetical protein -
FG175_RS25610 1068276..1068416 + 141 WP_154292170.1 hypothetical protein -
FG175_RS05060 1068413..1069126 + 714 WP_141958132.1 ABC transporter ATP-binding protein -
FG175_RS05065 1069123..1069980 + 858 WP_060440564.1 metal ABC transporter permease -
FG175_RS05070 1070006..1070884 + 879 WP_089185570.1 metal ABC transporter substrate-binding protein -
FG175_RS05075 1070991..1071131 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
FG175_RS05080 1071144..1071398 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T127704 WP_004940313.1 NZ_CP041134:c1067072-1066869 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T127704 NZ_CP041134:c1067072-1066869 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCGGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT127704 WP_004940312.1 NZ_CP041134:c1067491-1067123 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT127704 NZ_CP041134:c1067491-1067123 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGCATCGC
GACCCTGGGAGACAGCCACCACGGGTGGGTCAACGATCCGACATCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCAGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACTTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAGACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References