Detailed information of TA system TA127701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 14164..14765
• Replicon: chromosome
• Accession: NZ_CP041134
• Organism: Serratia marcescens strain WVU-010

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FG175_RS00070
• Protein ID: WP_060424343.1
• Coordinates: 14164..14544 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A086G6A0
• Locus tag: FG175_RS00075
• Protein ID: WP_004933932.1
• Coordinates: 14544..14765 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FG175_RS00045	9417..10697	+	1281	WP_055316928.1	DUF3748 domain-containing protein	-
FG175_RS00050	10728..11075	-	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
FG175_RS00055	11385..11798	+	414	WP_004933919.1	heat shock chaperone IbpA	-
FG175_RS00060	11899..12327	+	429	WP_004933922.1	heat shock chaperone IbpB	-
FG175_RS00065	12495..14153	+	1659	WP_084827017.1	putative transporter	-
FG175_RS00070	14164..14544	-	381	WP_060424343.1	type II toxin-antitoxin system VapC family toxin	Toxin
FG175_RS00075	14544..14765	-	222	WP_004933932.1	ribbon-helix-helix domain-containing protein	Antitoxin
FG175_RS00080	14841..16091	-	1251	WP_004933935.1	valine--pyruvate transaminase	-
FG175_RS00085	16227..18290	-	2064	WP_141957606.1	alpha-amylase	-
FG175_RS25595	18486..18638	+	153	WP_169317907.1	hypothetical protein	-
FG175_RS00090	18640..19617	-	978	WP_016929588.1	glyoxylate/hydroxypyruvate reductase GhrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14213.58 Da
• Isoelectric Point: 7.3170

>T127701 WP_060424343.1 NZ_CP041134:c14544-14164 [Serratia marcescens]
MVVQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T127701 NZ_CP041134:c14544-14164 [Serratia marcescens]
ATGGTGGTTCAACGGGCGCTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTCT
GACCGAATACCACATCAACCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATGGTGGGTGCCAAAAAACAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAACTACGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTACCGGCTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8593.53 Da
• Isoelectric Point: 4.4791

>AT127701 WP_004933932.1 NZ_CP041134:c14765-14544 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT127701 NZ_CP041134:c14765-14544 [Serratia marcescens]
ATGACCCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATTTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGTTATCTGACGGAGAACCACGTTGAAGATCCCAGCAATGCTTTCGGGCTCT
GGTCCGGGGCGATGGGCGATGGCGTAGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A086G6A0