Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 47646..48168 Replicon plasmid unnamed4
Accession NZ_CP041114
Organism Escherichia coli strain ECCTRSRTH03

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag CI675_RS24530 Protein ID WP_000220560.1
Coordinates 47887..48168 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag CI675_RS24525 Protein ID WP_000121743.1
Coordinates 47646..47897 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CI675_RS24485 43108..43338 + 231 WP_000051064.1 partitioning protein -
CI675_RS24490 43354..43647 + 294 WP_015060510.1 hypothetical protein -
CI675_RS24495 43649..44617 + 969 WP_077783213.1 IS5 family transposase -
CI675_RS24500 44677..45099 - 423 WP_031942371.1 DNA distortion polypeptide 3 -
CI675_RS24505 45167..45613 - 447 WP_001074386.1 hypothetical protein -
CI675_RS24510 45653..46489 - 837 WP_001050931.1 replication initiation protein -
CI675_RS24525 47646..47897 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
CI675_RS24530 47887..48168 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CI675_RS24535 48314..48637 + 324 WP_001181903.1 hypothetical protein -
CI675_RS24540 48682..48927 + 246 WP_000356546.1 hypothetical protein -
CI675_RS24545 48917..49132 + 216 WP_001180116.1 hypothetical protein -
CI675_RS24550 49225..49554 + 330 WP_000866648.1 hypothetical protein -
CI675_RS25005 49841..49990 + 150 WP_000003880.1 hypothetical protein -
CI675_RS24555 50003..50275 + 273 WP_000160399.1 hypothetical protein -
CI675_RS24560 50602..51147 + 546 WP_025755763.1 hypothetical protein -
CI675_RS24565 51150..52307 + 1158 WP_000538023.1 hypothetical protein -
CI675_RS24570 52668..53159 + 492 WP_000872475.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / mcr-3.5 - 1..58983 58983


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T127631 WP_000220560.1 NZ_CP041114:47887-48168 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T127631 NZ_CP041114:47887-48168 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT127631 WP_000121743.1 NZ_CP041114:47646-47897 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT127631 NZ_CP041114:47646-47897 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References