Detailed information of TA system TA127630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 53101..53623
• Replicon: plasmid unnamed3
• Accession: NZ_CP041113
• Organism: Escherichia coli strain ECCTRSRTH03

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: CI675_RS24125
• Protein ID: WP_000638823.1
• Coordinates: 53101..53382 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: CI675_RS24130
• Protein ID: WP_000121741.1
• Coordinates: 53372..53623 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI675_RS24090	49250..49570	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
CI675_RS24095	49641..49829	-	189	WP_050567133.1	hypothetical protein	-
CI675_RS24100	49931..50515	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
CI675_RS24105	50536..50934	-	399	WP_001153665.1	hypothetical protein	-
CI675_RS24110	51053..51490	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
CI675_RS24115	51496..52731	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
CI675_RS24120	52734..53033	-	300	WP_000835764.1	IncN plasmid KikA protein	-
CI675_RS24125	53101..53382	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CI675_RS24130	53372..53623	-	252	WP_000121741.1	hypothetical protein	Antitoxin
CI675_RS24135	53723..54358	-	636	WP_015059536.1	hypothetical protein	-
CI675_RS24140	54431..54718	-	288	WP_001032611.1	EexN family lipoprotein	-
CI675_RS24145	54731..54985	-	255	WP_001043555.1	EexN family lipoprotein	-
CI675_RS24150	55153..56184	+	1032	WP_001752311.1	IS630-like element ISEc33 family transposase	-
CI675_RS24155	56190..56783	-	594	WP_094316170.1	conjugal transfer protein TrbJ	-
CI675_RS24160	56789..57784	-	996	WP_001028543.1	type IV secretion system protein	-
CI675_RS24165	57788..58045	-	258	WP_000739144.1	hypothetical protein	-
CI675_RS24170	58042..58344	-	303	WP_001360345.1	hypothetical protein	-
CI675_RS24175	58325..58582	-	258	WP_001542015.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..63011	63011
	-	flank	IS/Tn	-	-	55153..56184	1031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T127630 WP_000638823.1 NZ_CP041113:c53382-53101 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T127630 NZ_CP041113:c53382-53101 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT127630 WP_000121741.1 NZ_CP041113:c53623-53372 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT127630 NZ_CP041113:c53623-53372 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1