Detailed information of TA system TA127629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 7032..7533
• Replicon: plasmid unnamed3
• Accession: NZ_CP041113
• Organism: Escherichia coli strain ECCTRSRTH03

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: CI675_RS23850
• Protein ID: WP_000702247.1
• Coordinates: 7032..7292 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: CI675_RS23855
• Protein ID: WP_001132407.1
• Coordinates: 7282..7533 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI675_RS23805	2628..2789	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
CI675_RS23810	2939..3535	+	597	WP_000125165.1	hypothetical protein	-
CI675_RS24980	3553..3837	-	285	WP_001162371.1	hypothetical protein	-
CI675_RS23820	3911..4576	-	666	WP_000532148.1	P-loop NTPase	-
CI675_RS23830	5174..5503	+	330	WP_000855120.1	hypothetical protein	-
CI675_RS23835	6085..6408	+	324	WP_000427403.1	hypothetical protein	-
CI675_RS23840	6450..6659	+	210	WP_000866036.1	hypothetical protein	-
CI675_RS23845	6756..6980	+	225	WP_000814381.1	hypothetical protein	-
CI675_RS23850	7032..7292	+	261	WP_000702247.1	hypothetical protein	Toxin
CI675_RS23855	7282..7533	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
CI675_RS23870	8083..8361	+	279	WP_000545938.1	hypothetical protein	-
CI675_RS23875	8781..9047	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
CI675_RS23880	9154..9336	-	183	WP_001481829.1	hypothetical protein	-
CI675_RS23885	9894..10076	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
CI675_RS23890	10101..10538	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
CI675_RS23895	10669..10995	-	327	WP_063078684.1	hypothetical protein	-
CI675_RS23900	11015..11722	-	708	WP_021503228.1	hypothetical protein	-
CI675_RS23905	11724..12476	-	753	WP_000797966.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..63011	63011

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T127629 WP_000702247.1 NZ_CP041113:7032-7292 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T127629 NZ_CP041113:7032-7292 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT127629 WP_001132407.1 NZ_CP041113:7282-7533 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT127629 NZ_CP041113:7282-7533 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0