Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 36096..36643 Replicon plasmid unnamed2
Accession NZ_CP041112
Organism Escherichia coli strain ECCTRSRTH03

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag CI675_RS23430 Protein ID WP_000969996.1
Coordinates 36362..36643 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1M1SX19
Locus tag CI675_RS23425 Protein ID WP_021546940.1
Coordinates 36096..36365 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CI675_RS23395 31590..31829 + 240 Protein_36 RepA protein -
CI675_RS23400 31875..32572 + 698 Protein_37 IS1 family transposase -
CI675_RS23405 32913..33902 + 990 WP_001515196.1 tyrosine-type recombinase/integrase -
CI675_RS23425 36096..36365 + 270 WP_021546940.1 hypothetical protein Antitoxin
CI675_RS23430 36362..36643 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CI675_RS23435 36689..36901 + 213 Protein_42 3'-5' exonuclease -
CI675_RS23440 36975..37184 + 210 Protein_43 transposase -
CI675_RS23445 37485..39236 - 1752 Protein_44 Tn3-like element TnAs1 family transposase -
CI675_RS23450 39225..39632 + 408 WP_001214976.1 cysteine hydrolase -
CI675_RS23455 39770..40654 + 885 WP_000058717.1 EamA family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / ant(3'')-Ia / blaTEM-1B / tet(M) / aph(3')-Ia / sul3 / cmlA1 / aadA2 / dfrA12 / aph(3'')-Ib / aph(6)-Id - 1..87516 87516
- inside IScluster/Tn tet(A) / ant(3'')-Ia / blaTEM-1B / tet(M) / aph(3')-Ia / sul3 / cmlA1 / aadA2 / dfrA12 / aph(3'')-Ib / aph(6)-Id - 31875..86605 54730


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T127628 WP_000969996.1 NZ_CP041112:36362-36643 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T127628 NZ_CP041112:36362-36643 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCATCCCCATTTTGTTCTGGTTTATGAAGTCGATAGCCAGTGGGGTAAAGTG
TATATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10321.69 Da        Isoelectric Point: 8.8725

>AT127628 WP_021546940.1 NZ_CP041112:36096-36365 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMRRKVADK

Download         Length: 270 bp

>AT127628 NZ_CP041112:36096-36365 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGTGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGCGCAGGAAGGTTGCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1SX19

References