Detailed information of TA system TA127601

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 768662..769494
• Replicon: chromosome
• Accession: NZ_CP041110
• Organism: Escherichia coli strain ECCTRSRTH03

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CI675_RS03775
• Protein ID: WP_000854816.1
• Coordinates: 768662..769036 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CI675_RS03780
• Protein ID: WP_094316153.1
• Coordinates: 769126..769494 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI675_RS03740	763690..764466	+	777	Protein_733	Accessory colonization factor AcfD	-
CI675_RS03745	764466..765035	+	570	Protein_734	type II secretion system minor pseudopilin GspK	-
CI675_RS03750	765032..766210	+	1179	WP_000094991.1	type II secretion system protein GspL	-
CI675_RS03755	766212..766748	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
CI675_RS03760	767030..767872	-	843	WP_001274564.1	DUF4942 domain-containing protein	-
CI675_RS03765	767957..768154	-	198	WP_000772027.1	DUF957 domain-containing protein	-
CI675_RS03770	768174..768665	-	492	WP_000976865.1	hypothetical protein	-
CI675_RS03775	768662..769036	-	375	WP_000854816.1	type IV toxin-antitoxin system toxin CbtA	Toxin
CI675_RS03780	769126..769494	-	369	WP_094316153.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CI675_RS03785	769657..769878	-	222	WP_000692323.1	DUF987 domain-containing protein	-
CI675_RS03790	769947..770423	-	477	WP_001186727.1	RadC family protein	-
CI675_RS03795	770439..770918	-	480	WP_094316154.1	antirestriction protein	-
CI675_RS03800	771181..771999	-	819	WP_001234639.1	DUF945 domain-containing protein	-
CI675_RS24795	772090..772299	-	210	Protein_746	DUF905 family protein	-
CI675_RS03815	772400..772855	-	456	WP_024226900.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14045.05 Da
• Isoelectric Point: 9.1493

>T127601 WP_000854816.1 NZ_CP041110:c769036-768662 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAK

Nucleotide

• Length: 375 bp

>T127601 NZ_CP041110:c769036-768662 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCGCGTCCGTCTCCTGTTGAAATCTGGCAGACACTGCTGTC
CCGCCTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCCCGGTTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13899.62 Da
• Isoelectric Point: 6.3165

>AT127601 WP_094316153.1 NZ_CP041110:c769494-769126 [Escherichia coli]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGSRLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELILTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT127601 NZ_CP041110:c769494-769126 [Escherichia coli]
GTGTCAGACACGCACCATGAGACAAATTATCCCGACGACTATAACAACCGCCCCTGGTGGGGGCTGCCCTGCACCATAAC
ACCCTGTTTTGGCTCCCGTCTGGTGCAGGAGGGTAACCGGTTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATACTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGACTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA